公开(公告)号：US12077786B2

公开(公告)日：2024-09-03

申请号：US16966768

申请日：2019-02-01

Applicant: LONZA LTD

Inventor： Robert Young ,  Christopher Mark Smales ,  Colin Mark Jaques ,  Andrew J. Racher ,  Gurdeep Singh ,  James Budge ,  Joanne Robool

IPC: C12N9/06 ,  C12N9/02 ,  C12N15/10 ,  C12N15/63

CPC classification number: C12N9/0008 ,  C12N15/1075 ,  C12N15/63

Abstract: Described herein are compositions and methods for identifying, selecting, or culturing cells comprising a subject nucleic acid sequence of interest. Generally, a nucleic acid comprising a subject nucleic acid and a sequence encoding an enzyme molecule involved in biosynthesis of an amino acid is introduced into a cell. The cell is then grown on media lacking the amino acid, such that cells comprising the introduced nucleic acid are capable of growth. In some instances, the cell further comprises an inhibitor of the enzyme molecule to increase the stringency of the selection.

公开(公告)号：US20170321194A1

公开(公告)日：2017-11-09

申请号：US15585570

申请日：2017-05-03

Applicant: LONZA LTD

Inventor： James Budge ,  Christopher Mark Smales ,  Tanya Jeane Knight ,  Robert Young

IPC: C12N9/02 ,  C07K16/00 ,  C12N5/071 ,  C07K14/47

CPC classification number: C12N9/0097 ,  C07K14/4702 ,  C07K16/00 ,  C07K2317/14 ,  C12N5/0682 ,  C12N9/0071 ,  C12N9/0083 ,  C12P21/02 ,  C12Y114/19001

Abstract: The present disclosure features methods and compositions for modulating lipid metabolism to achieve improved production and quality of recombinant products, such as next generation biologics. Modulation of lipid metabolism as described herein includes, for example, introducing a lipid metabolism modulator described herein to a cell or a cell-free system. Also encompassed by the present disclosure are engineered cells with improved production capacity and improved product quality, methods for engineering such cells, and preparations and mixtures comprising the products from such cells.

公开(公告)号：US12054733B2

公开(公告)日：2024-08-06

申请号：US16622509

申请日：2018-06-15

Applicant: LONZA LTD

Inventor： Robert Young ,  Peter Michael O'Callaghan ,  Thomas Payne

IPC: C12N15/85 ,  C12N9/22 ,  C12N15/11 ,  A61K39/00

CPC classification number: C12N15/85 ,  C12N9/22 ,  C12N15/11 ,  A61K39/00 ,  C12N2800/80

Abstract: Disclosed are genetic control circuits, cells, and methods that use a repressor polypeptide to reduce the transcription rate of an exogenous therapeutic polypeptide encoding gene in response to a change in condition.

公开(公告)号：US20200231944A1

公开(公告)日：2020-07-23

申请号：US16818162

申请日：2020-03-13

Applicant: LONZA LTD

Inventor： James Budge ,  Christopher Mark Smales ,  Tanya Jeane Knight ,  Robert Young

IPC: C12N9/02 ,  C12P21/02 ,  C07K14/47 ,  C07K16/00 ,  C12N5/071

Abstract: The present disclosure features methods and compositions for modulating lipid metabolism to achieve improved production and quality of recombinant products, such as next generation biologics. Modulation of lipid metabolism as described herein includes, for example, introducing a lipid metabolism modulator described herein to a cell or a cell-free system. Also encompassed by the present disclosure are engineered cells with improved production capacity and improved product quality, methods for engineering such cells, and preparations and mixtures comprising the products from such cells.

公开(公告)号：US11434474B2

公开(公告)日：2022-09-06

申请号：US16818162

申请日：2020-03-13

Applicant: LONZA LTD

Inventor： James Budge ,  Christopher Mark Smales ,  Tanya Jeane Knight ,  Robert Young

IPC: C12N15/87 ,  C12N9/02 ,  C12P21/02 ,  C07K14/47 ,  C07K16/00 ,  C12N5/071

Abstract: The present disclosure features methods and compositions for modulating lipid metabolism to achieve improved production and quality of recombinant products, such as next generation biologics. Modulation of lipid metabolism as described herein includes, for example, introducing a lipid metabolism modulator described herein to a cell or a cell-free system. Also encompassed by the present disclosure are engineered cells with improved production capacity and improved product quality, methods for engineering such cells, and preparations and mixtures comprising the products from such cells.

公开(公告)号：US10655111B2

公开(公告)日：2020-05-19

申请号：US15585570

申请日：2017-05-03

Applicant: LONZA LTD

Inventor： James Budge ,  Christopher Mark Smales ,  Tanya Jeane Knight ,  Robert Young

IPC: C07K16/00 ,  C12N9/02 ,  C12P21/02 ,  C07K14/47 ,  C12N5/071

Abstract: The present disclosure features methods and compositions for modulating lipid metabolism to achieve improved production and quality of recombinant products, such as next generation biologics. Modulation of lipid metabolism as described herein includes, for example, introducing a lipid metabolism modulator described herein to a cell or a cell-free system. Also encompassed by the present disclosure are engineered cells with improved production capacity and improved product quality, methods for engineering such cells, and preparations and mixtures comprising the products from such cells.