公开(公告)号：US20230143960A1

公开(公告)日：2023-05-11

申请号：US17981943

申请日：2022-11-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： William LAFFERTY ,  Mikhail Bashtanov ,  Peter Crossley ,  Abi Graham ,  Gary Jepps ,  Richard Lintern ,  Stuart Lowe ,  Yang Lu ,  James Onslow ,  Neil Pollock ,  Sebastian Rammensee ,  Richard Janse van Rensburg ,  Steve Wakefield ,  Peter Eugster

IPC: B01L3/00 ,  C12Q1/686

CPC classification number: B01L3/502 ,  B01L3/5027 ,  C12Q1/686 ,  B01L2300/123 ,  B01L2400/043 ,  B01L7/52

Abstract: A cartridge can comprise a first elastomeric membrane and a second elastomeric membrane, and portions of the elastomeric membranes which are sealed to each other can circumscribe unsealed portions of the membranes. In a resting state, the unsealed portion of the first elastomeric membrane abuts or is proximate to the unsealed portion of the second elastomeric membrane. One or more reagents can be injected between the unsealed portions of the first and second elastomeric membranes to push the unsealed portions apart from each other in this region of the membranes. The unsealed portions can be sequentially pushed apart in downstream regions to form a channel between the elastomeric membranes. Positively displaced fluid pushes the unsealed membrane portions apart to a volume that conforms to the volume of the fluid to minimize or prevent dead volume in the channel and thus minimize or prevent air bubbles in the fluid.