公开(公告)号：US10371604B2

公开(公告)日：2019-08-06

申请号：US15233801

申请日：2016-08-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mousumi Rath ,  Mark Shannon ,  Jason La

IPC: G01N1/04 ,  C12Q1/6806 ,  G01N1/28 ,  G01N1/34 ,  G01N1/02 ,  B01L3/00

Abstract: In one embodiment, a method for processing a sample includes selecting a selected sample from a biological specimen, the selected sample being in contact with a first surface of a first substrate. The method also includes transferring the selected sample directly from the first surface to a container comprising an internal volume. The method also includes forming or providing a sample solution within the internal volume of the container by contacting the selected sample with a lysis mixture using a protocol. The method further includes performing an assay, experiment, or test on the sample solution while the sample solution disposed is within the internal volume of the container.In another embodiment, a method for processing a sample includes providing a selected sample comprising one or more cells. The method also includes transferring the selected sample into an internal volume of a container. The method also includes contacting the selected sample with a lysis mixture using a protocol to provide a sample solution, wherein the protocol comprises heating the sample solution to a first temperature that is greater than 37 degrees Celsius and less than or equal to 75 degrees Celsius.