公开(公告)号：US20170219522A1

公开(公告)日：2017-08-03

申请号：US15420496

申请日：2017-01-31

Applicant: LI-COR, Inc.

Inventor： Michael D. Furtaw ,  Donald T. Lamb

IPC: G01N27/447

Abstract: Devices, systems, and methods of using them are disclosed that position an end of a capillary electrophoresis tube within an internal tapered nozzle region of an inkjet print head or other microfluidic pump. The capillary electrophoresis tube can extend through an inlet of the microfluidic pump and leave space for a sheath liquid to enter the pump and mix with separated analytes eluted from the capillary electrophoresis tube. The small volume of mixed sheath liquid and analyte can then be jetted through the nozzle at a moving surface, either continuously or as discrete droplets. Relative positions on the surface can indicate separation distances of dispensed analytes.

公开(公告)号：US20220057360A1

公开(公告)日：2022-02-24

申请号：US17518430

申请日：2021-11-03

Applicant: LI-COR, Inc.

Inventor： Michael D. Furtaw ,  Donald T. Lamb

IPC: G01N27/447

Abstract: A flat bar piezoelectric actuator affixed to a pressure chamber with one or more separation capillary tubes exiting near respective nozzle orifices is disclosed. The flat actuator against a flat wall of the pump chamber causes a relatively planar pressure wave to pass by the end of each capillary, transporting a precise amount of separated analyte from the capillary out of the nozzle orifice. The nozzle may or may not be tapered. Multiple nozzles can form an inkjet print head that ejects precise droplets of analyte and sheath fluid. The small volume of mixed sheath liquid and analyte can then be jetted through the nozzle at a moving surface, either continuously or as discrete droplets. Relative positions on the surface can indicate separation distances of dispensed analytes.

公开(公告)号：US11241689B2

公开(公告)日：2022-02-08

申请号：US15670896

申请日：2017-08-07

Applicant: LI-COR, Inc.

Inventor： Michael D. Furtaw ,  Donald T. Lamb

IPC: G01N27/447 ,  B01L3/00

Abstract: Devices and methods are provided for the separation and dispensing of material using a microfluidic electrophoresis column, sheath liquid pump, and exit channel, all on the same monolithic chip. Material is separated in the electrophoresis column and passed into the exit chamber in response to a voltage potential between a first electrode within the electrophoresis column and a terminating electrode integrated into the chip. The terminating electrode can be in the sheath liquid pump chamber, the sheath liquid reservoir, or a separate flow channel that intersects the exit channel along with the electrophoresis column and sheath liquid pump chamber. The flow of sheath liquid into the exit chamber entrains separated analytes into an effluent that is dispensed out of the exit chamber via a discharge outlet.

公开(公告)号：US11154862B2

公开(公告)日：2021-10-26

申请号：US16876895

申请日：2020-05-18

Applicant: LI-COR, Inc.

Inventor： Michael D. Furtaw ,  Donald T. Lamb ,  Lyle R. Middendorf

IPC: G01N27/447 ,  B01L3/00 ,  B01L3/02 ,  G01N1/40

Abstract: Devices and methods are provided for the separation and dispensing of material using a microfluidic separation column connected via an exit channel to one or more sheath flow channels. The flow of separated material through the separation column is at least partially driven by a voltage potential between a first electrode within the separation column and a terminating electrode within at least one of the sheath flow channels. The separation column, exit channel, sheath flow channels, and electrodes are all within a single monolithic chip. The presence of an on-chip terminating electrode allows for separated material to be entrained in the sheath fluids and ejected onto a surface that can be non-conductive. The presence of multiple sheath flows allows for sheath flow fluids to have different compositions from one another, while reducing the occurrence of sheath flow fluids entering the separation column.

公开(公告)号：US10670560B2

公开(公告)日：2020-06-02

申请号：US15420496

申请日：2017-01-31

Applicant: LI-COR, Inc.

Inventor： Michael D. Furtaw ,  Donald T. Lamb

IPC: G01N27/447

Abstract: Devices, systems, and methods of using them are disclosed that position an end of a capillary electrophoresis tube within an internal tapered nozzle region of an inkjet print head or other microfluidic pump. The capillary electrophoresis tube can extend through an inlet of the microfluidic pump and leave space for a sheath liquid to enter the pump and mix with separated analytes eluted from the capillary electrophoresis tube. The small volume of mixed sheath liquid and analyte can then be jetted through the nozzle at a moving surface, either continuously or as discrete droplets. Relative positions on the surface can indicate separation distances of dispensed analytes.

公开(公告)号：US20180036730A1

公开(公告)日：2018-02-08

申请号：US15670939

申请日：2017-08-07

Applicant: LI-COR, Inc.

Inventor： Michael D. Furtaw ,  Donald T. Lamb ,  Lyle R. Middendorf

IPC: B01L3/00 ,  G01N27/447 ,  G01N1/40

Abstract: Devices and methods are provided for the separation and dispensing of material using a microfluidic separation column connected via an exit channel to one or more sheath flow channels. The flow of separated material through the separation column is at least partially driven by a voltage potential between a first electrode within the separation column and a terminating electrode within at least one of the sheath flow channels. The separation column, exit channel, sheath flow channels, and electrodes are all within a single monolithic chip. The presence of an on-chip terminating electrode allows for separated material to be entrained in the sheath fluids and ejected onto a surface that can be non-conductive. The presence of multiple sheath flows allows for sheath flow fluids to have different compositions from one another, while reducing the occurrence of sheath flow fluids entering the separation column.

公开(公告)号：US20180036729A1

公开(公告)日：2018-02-08

申请号：US15670896

申请日：2017-08-07

Applicant: LI-COR, Inc.

Inventor： Michael D. Furtaw ,  Donald T. Lamb

IPC: B01L3/00 ,  G01N27/447

CPC classification number: B01L3/50273 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2400/0421 ,  B01L2400/0478 ,  G01N27/44721 ,  G01N27/44739 ,  G01N27/44773 ,  G01N27/44782 ,  G01N27/44791

Abstract: Devices and methods are provided for the separation and dispensing of material using a microfluidic electrophoresis column, sheath liquid pump, and exit channel, all on the same monolithic chip. Material is separated in the electrophoresis column and passed into the exit chamber in response to a voltage potential between a first electrode within the electrophoresis column and a terminating electrode integrated into the chip. The terminating electrode can be in the sheath liquid pump chamber, the sheath liquid reservoir, or a separate flow channel that intersects the exit channel along with the electrophoresis column and sheath liquid pump chamber. The flow of sheath liquid into the exit chamber entrains separated analytes into an effluent that is dispensed out of the exit chamber via a discharge outlet.

公开(公告)号：US20220219166A1

公开(公告)日：2022-07-14

申请号：US17575275

申请日：2022-01-13

Applicant: LI-COR, Inc.

Inventor： Michael D. Furtaw ,  Donald T. Lamb

IPC: B01L3/00 ,  G01N27/447

Abstract: A multilayered microfluidic chip integrating separation channels and a common piezoelectric pump dispensing to a blotting membrane is described. A top layer with separation channels is connected with vias through a middle layer to a nozzle area in a bottom layer that has a piezoelectric pump. Because each via is very near a separate orifice in the bottom layer, the buffer fluid in the bottom layer will quickly dispense analyte emerging from the via. The analyte is pumped out of the orifice carried by the buffer fluid. A common reservoir of buffer fluid, connected with the pump membrane, is used. Electrodes may be placed near the entrance of each separation channel and share a terminating electrode in the common reservoir.

公开(公告)号：US11255816B2

公开(公告)日：2022-02-22

申请号：US16860514

申请日：2020-04-28

Applicant: LI-COR, Inc.

Inventor： Michael D. Furtaw ,  Donald T. Lamb

IPC: G01N27/447

Abstract: Devices, systems, and methods of using them are disclosed that position an end of a capillary electrophoresis tube within an internal tapered nozzle region of an inkjet print head or other microfluidic pump. The capillary electrophoresis tube can extend through an inlet of the microfluidic pump and leave space for a sheath liquid to enter the pump and mix with separated analytes eluted from the capillary electrophoresis tube. The small volume of mixed sheath liquid and analyte can then be jetted through the nozzle at a moving surface, either continuously or as discrete droplets. Relative positions on the surface can indicate separation distances of dispensed analytes.

公开(公告)号：US20200276583A1

公开(公告)日：2020-09-03

申请号：US16876895

申请日：2020-05-18

Applicant: LI-COR, Inc.

Inventor： Michael D. Furtaw ,  Donald T. Lamb ,  Lyle R. Middendorf

IPC: B01L3/00 ,  B01L3/02 ,  G01N27/447 ,  G01N1/40

Abstract: Devices and methods are provided for the separation and dispensing of material using a microfluidic separation column connected via an exit channel to one or more sheath flow channels. The flow of separated material through the separation column is at least partially driven by a voltage potential between a first electrode within the separation column and a terminating electrode within at least one of the sheath flow channels. The separation column, exit channel, sheath flow channels, and electrodes are all within a single monolithic chip. The presence of an on-chip terminating electrode allows for separated material to be entrained in the sheath fluids and ejected onto a surface that can be non-conductive. The presence of multiple sheath flows allows for sheath flow fluids to have different compositions from one another, while reducing the occurrence of sheath flow fluids entering the separation column.