公开(公告)号：US20230400411A1

公开(公告)日：2023-12-14

申请号：US18331969

申请日：2023-06-09

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Irmtraud STEINMETZ ,  Luis ALVAREZ

IPC: G01N21/64

CPC classification number: G01N21/6458 ,  G01N2201/06113 ,  G01N2021/6419 ,  G01N2021/6421

Abstract: A controller for an imaging device is configured to control an optical detection unit to receive fluorescence light emitted by first and second fluorophore species excited by first and second excitation light, respectively, to separate the fluorescence light into at least two spectral detection channels corresponding to two wavelength bands, respectively. A first wavelength band includes an emission spectrum of the first fluorophore species. A second wavelength band includes an emission spectrum of the second fluorophore species. The controller is further configured to control the optical detection unit to detect photon arrival times of the fluorescence light relative to light pulses of the second excitation light, to determine a temporal correlation between the light pulses and the photon arrival times, and to determine a first number and a second number of photons received in the first spectral detection channel and/or the second spectral detection channel based on the temporal correlation.

公开(公告)号：US20250031462A1

公开(公告)日：2025-01-23

申请号：US18774962

申请日：2024-07-17

Applicant: Leica Microsystems CMS GmbH

Inventor： Luis ALVAREZ ,  Ulf SCHWARZ ,  Florian EICH ,  Giulia OSSATO

IPC: H01L27/144 ,  H01L27/146

Abstract: A detector device for a microscope includes a multi-element photodetector having a plurality of photodetector elements arranged in a photodetector array. Each photodetector element is configured to output a detector signal upon receiving light. The plurality of photodetector elements is arranged in one or more photodetector groups. Each photodetector group has a signal combiner configured to combine the detector signals of the photodetector elements into a collective output signal of the photodetector group to reduce a dead time thereof. In a case of only one photodetector group, the multi-element photodetector includes an optical distributor configured to distribute the light across the photodetector group; or in a case of more than one photodetector group, the photodetector groups differ from each other with respect to a density at which the photodetector elements are arranged in the respective photodetector group.

公开(公告)号：US20220343467A1

公开(公告)日：2022-10-27

申请号：US17636035

申请日：2020-10-08

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Luis ALVAREZ ,  Frank HECHT

IPC: G06T5/00 ,  G06T5/50 ,  G06T5/20 ,  G06T5/10 ,  G01N21/64

Abstract: The present invention relates to a method for generating a result image. The method comprises the steps of: acquiring a STED image of a sample, the STED image comprising pixels; calculating Fourier coefficients of arrival times for the pixels of the image, resulting in real coefficients representing a first image and in imaginary coefficients representing a second image; deriving an intensity image from the STED image; applying a spatial filter to the first image, the second image and the intensity image, resulting in a filtered first image, a filtered second image, and filtered intensity image, respectively; calculating an image G based on the filtered first image and the filtered intensity image; calculating an image S based on the filtered second image and the filtered intensity image; and calculating a result image based on the image G and the image S.

公开(公告)号：US20240290117A1

公开(公告)日：2024-08-29

申请号：US18586622

申请日：2024-02-26

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Luis ALVAREZ ,  Frank HECHT ,  Julia ROBERTI ,  Giulia OSSATO

IPC: G06V20/69 ,  G01N21/64 ,  G06V10/25

CPC classification number: G06V20/698 ,  G01N21/6408 ,  G01N21/6458 ,  G06V10/25 ,  G06V20/693 ,  G06V20/695

Abstract: A processor for lifetime-based unmixing in fluorescence microscopy is configured to acquire an image having a plurality of pixels, each pixel providing information on photon count and photon arrival times, generate a phasor plot that is a vector space representation of the image, partition the image into image segments, evaluate the image segments according to total photon counts of the corresponding subsets of pixels, and execute a lifetime classification by selecting an image segment having a largest total photon count, determining a region of interest in the image encompassing the image segment, determining a phasor subset in the phasor plot corresponding to the region of interest, and generating a lifetime class including a set of image segments corresponding to the phasor subset. A plurality of lifetime classes is generated by iteratively executing the lifetime classification. The processor is configured to perform lifetime-based unmixing using the life-time classes.