公开(公告)号：US20150002917A1

公开(公告)日：2015-01-01

申请号：US14485153

申请日：2014-09-12

Applicant: Leica Microsystems CMS GmbH

Inventor： Rolf BORLINGHAUS ,  Irmtraud STEINMETZ

IPC: G02B21/06 ,  G02B26/02

CPC classification number: G02B21/06 ,  G01J3/0208 ,  G01J3/0237 ,  G01J3/04 ,  G01J3/14 ,  G01J3/36 ,  G02B5/005 ,  G02B21/00 ,  G02B21/0024 ,  G02B26/02

Abstract: A microscope has an aperture arrangement that, in order to limit the dimension of a light beam, comprises an aperture opening. The size of the aperture opening is adjustable with the aid of a first aperture member and a second aperture member. At least one of the two aperture members is movable relative to the other aperture member. The aperture members are spaced apart from one another when the aperture opening is closed.

Abstract translation: 显微镜具有孔径布置，为了限制光束的尺寸，其包括孔口。 借助于第一孔径构件和第二孔径构件可以调整孔径开口的尺寸。 两个光阑元件中的至少一个可相对于另一个光阑元件移动。 当孔开口关闭时，孔部件彼此间隔开。

公开(公告)号：US20230400411A1

公开(公告)日：2023-12-14

申请号：US18331969

申请日：2023-06-09

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Irmtraud STEINMETZ ,  Luis ALVAREZ

IPC: G01N21/64

CPC classification number: G01N21/6458 ,  G01N2201/06113 ,  G01N2021/6419 ,  G01N2021/6421

Abstract: A controller for an imaging device is configured to control an optical detection unit to receive fluorescence light emitted by first and second fluorophore species excited by first and second excitation light, respectively, to separate the fluorescence light into at least two spectral detection channels corresponding to two wavelength bands, respectively. A first wavelength band includes an emission spectrum of the first fluorophore species. A second wavelength band includes an emission spectrum of the second fluorophore species. The controller is further configured to control the optical detection unit to detect photon arrival times of the fluorescence light relative to light pulses of the second excitation light, to determine a temporal correlation between the light pulses and the photon arrival times, and to determine a first number and a second number of photons received in the first spectral detection channel and/or the second spectral detection channel based on the temporal correlation.

公开(公告)号：US20130170023A1

公开(公告)日：2013-07-04

申请号：US13753161

申请日：2013-01-29

Applicant: Leica Microsystems CMS GmbH

Inventor： Rolf BORLINGHAUS ,  Irmtraud STEINMETZ

IPC: G02B21/00 ,  G02B21/06

CPC classification number: G02B21/06 ,  G01J3/0208 ,  G01J3/0237 ,  G01J3/04 ,  G01J3/14 ,  G01J3/36 ,  G02B5/005 ,  G02B21/00 ,  G02B21/0024 ,  G02B26/02

Abstract: A microscope (10) has an aperture arrangement (29) that, in order to limit the dimension of a light beam (41), comprises an aperture opening (37). The size of the aperture opening (37) is adjustable with the aid of a first aperture member (32) and a second aperture member (34). At least one of the two aperture members (32, 34) is movable relative to the other aperture member (32, 34). The aperture members (32, 34) are spaced apart from one another when the aperture opening (37) is closed.

Abstract translation: 显微镜（10）具有孔径布置（29），为了限制光束（41）的尺寸，其包括孔口（37）。 借助于第一孔径构件（32）和第二孔口构件（34），孔口（37）的尺寸是可调节的。 两个孔径构件（32,34）中的至少一个可相对于另一个孔构件（32,34）移动。 当孔口（37）关闭时，孔部件（32,34）彼此间隔开。