公开(公告)号：US20230147321A1

公开(公告)日：2023-05-11

申请号：US17918348

申请日：2021-03-29

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Jonas FOELLING

IPC: G02B6/42

CPC classification number: G02B6/4214

Abstract: A system for coupling at least one optical beam includes at least one optical beam entry port, an optical beam exit port, the optical beam exit port including a lens performing a Fourier Transform, at least one optical beam deflector, and an optical base element, wherein the at least one directly fixed optical beam deflector is allowed to rotate around a rotation axis, wherein the corresponding at least one optical beam and/or its assigned optical beam entry port is configured such that the semi-major axis of the elliptical cross section of the optical beam on a deflection surface of a respective optical beam deflector is oriented parallel to the rotation axis, and wherein, after having passed the optical beam exit port, the elliptical cross section of the at least one optical beam overlaps the circular cross section of the light receiver.

公开(公告)号：US20230008453A1

公开(公告)日：2023-01-12

申请号：US17779592

申请日：2020-11-18

Applicant: Leica Microsystems CMS GmbH

Inventor： Arnold GISKE ,  Jonas FOELLING

IPC: G02B21/00 ,  G01N21/64

Abstract: A laser scanning microscope includes a light source configured to emit an illumination light beam. The illumination light beam has a transverse light intensity profile comprising an intensity minimum. The laser scanning microscope further includes a scanning device configured to scan the illumination light beam along a closed trajectory in a target area of a specimen, and a detector configured to detect fluorescence light emitted by a fluorophore within the target area of the specimen. The fluorophore is excited by the illumination light beam. The laser scanning microscope further includes a processor configured to determine an intensity distribution of the fluorescence light as a function of time and to determine a position of the fluorophore within the target area based on the intensity distribution of the fluorescence light.

公开(公告)号：US20130119273A1

公开(公告)日：2013-05-16

申请号：US13668414

申请日：2012-11-05

Applicant: Leica Microsystems CMS GmbH

Inventor： Jonas FOELLING

IPC: G01N21/64

CPC classification number: G01N21/6408 ,  G01N21/6458 ,  G02B21/06 ,  G02B21/16

Abstract: A method for illumination and detection in RESOLFT microscopy using a pulsed or continuous light source for excitation light and switching light is characterized in that the excitation light (4) is irradiated in pulses and in that the pulse of the excitation light (4) is longer than 150 picoseconds, preferably up to a few hundred picoseconds, and even up to a few nanoseconds. A corresponding apparatus uses the method according to the present invention.

Abstract translation: 用于激发光和切换光的脉冲或连续光源的RESOLFT显微镜中的照明和检测方法的特征在于激发光（4）以脉冲照射，并且激发光（4）的脉冲更长 超过150皮秒，优选高达几百皮秒，甚至高达几纳秒。 相应的装置使用根据本发明的方法。

公开(公告)号：US20220196555A1

公开(公告)日：2022-06-23

申请号：US17603596

申请日：2020-04-16

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Jonas FOELLING ,  Lars FRIEDRICH

IPC: G01N21/64

Abstract: A fluorescence microscope includes excitation and de-excitation light sources designed to generate excitation and de-excitation light distributions, which excite and de-excite fluorophores present in a sample, respectively. An illumination unit is designed to combine the light distributions such that an intensity maximum of the excitation light distribution and an intensity minimum of the de-excitation light distribution are spatially superimposed on one another in an illumination target point. A detector is designed to detect the fluorescence photons as a function of their arrival times. The processor is designed to evaluate the detected fluorescence photons with respect to their arrival times and, based thereon, to control a delay which a light pulse or a light modulation of the de-excitation light distribution has at a position of the illumination target point in relation to a light pulse or a light modulation of the excitation light distribution.

公开(公告)号：US20220196554A1

公开(公告)日：2022-06-23

申请号：US17603594

申请日：2020-04-17

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Jonas FOELLING ,  Lars FRIEDRICH

IPC: G01N21/64

Abstract: A fluorescence scanning microscope includes excitation and de-excitation light sources, which are designed to generate an excitation and a de-excitation light distribution, respectively. An illumination unit combines the light distributions to form a light distribution scanning over multiple illumination target points of a sample in such a way that an intensity maximum of the excitation light distribution and an intensity minimum of the de-excitation light distribution are spatially superimposed on one another. A detector detects fluorescence photons emitted from the respective illumination target point as a function of their arrival times. A processor evaluates the fluorescence photons with respect to the arrival times, generates a first pixel and a second pixel based thereon, assembles the first and second pixels to form first and second sample images, respectively, and, by means of the two sample images, determines a spatial offset between the intensity maximum and the intensity minimum.

公开(公告)号：US20160063718A1

公开(公告)日：2016-03-03

申请号：US14778638

申请日：2014-03-24

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Jonas FOELLING

IPC: G06T7/00 ,  G06K9/46 ,  G02B21/18 ,  G02B3/06 ,  G02B21/36 ,  G02B27/00

CPC classification number: G06T7/55 ,  G01N21/6458 ,  G02B3/06 ,  G02B21/16 ,  G02B21/18 ,  G02B21/361 ,  G02B21/367 ,  G02B27/0075 ,  G02B27/58 ,  G06K9/4671 ,  G06T2207/10028 ,  G06T2207/10056 ,  G06T2207/30004 ,  G06T2207/30204

Abstract: A light-microscopy method for locating point objects in a sample arranged in an object space includes imaging the sample onto a detector by an imaging optical unit having a depth of field of predetermined axial extent along an optical axis in the object space, onto which the detector is imaged. The point objects in the sample are located within the depth of field. The first sample image generated by the imaging of the sample onto the detector is evaluated. For locating a respective first point object in a direction of the optical axis, a parameter of a first light spot of one or more light spots of the first sample image representing the first point object is determined, and a rough axial z position related to the first point object is assigned to the parameter based on predetermined association information.

Abstract translation: 用于定位在对象空间中的样本中的点对象的光学显微镜方法包括：通过成像光学单元将样本成像到检测器上，所述成像光学单元沿对象空间中的光轴具有预定轴向长度的景深， 检测器成像。 样本中的点对象位于景深范围内。 评估通过将样品成像到检测器上而产生的第一样本图像。 为了在光轴的方向上定位相应的第一点对象，确定表示第一点对象的第一样本图像中的一个或多个光点的第一光点的参数，以及与该第一点对象相关的粗略轴向z位置 基于预定的关联信息将第一点对象分配给参数。