公开(公告)号：US20040043507A1

公开(公告)日：2004-03-04

申请号：US10228838

申请日：2002-08-27

Applicant: Kimberly-Clark Worldwide, Inc.

Inventor： Xuedong Song ,  Rosann Kaylor ,  Shawn Feaster ,  Kaiyuan Yang

IPC: G01N033/553

CPC classification number: G01N33/54366 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0825 ,  B01L2400/0406 ,  B01L2400/043 ,  B01L2400/0677 ,  G01N33/54333 ,  Y10S435/969 ,  Y10S435/97 ,  Y10T436/10

Abstract: A fluidics-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes a self-calibrated magnetic binding assay format (e.g., sandwich, competitive, etc.) that includes detection probes capable of generating a detection signal (e.g., fluorescent non-magnetic particles) and calibration probes capable of generating a calibration signal (e.g., fluorescent magnetic particles). The amount of the analyte within the test sample is proportional (e.g., directly or inversely) to the intensity of the detection signal calibrated by the intensity of the calibration signal. It has been discovered that the fluidics-based device of the present invention provides an accurate, inexpensive, and readily controllable method of determining the presence of an analyte in a test sample.

Abstract translation: 提供了一种用于检测驻留在测试样品中的分析物的存在或数量的基于流体学的测定装置。 该装置使用包括能够产生检测信号的检测探针（例如，荧光非磁性颗粒）和能够产生校准信号的校准探针的自校准磁结合测定格式（例如，夹层，竞争性等） 例如荧光磁性颗粒）。 测试样品内的分析物的量与由校准信号的强度校准的检测信号的强度成正比（例如，直接或反向）。 已经发现，本发明的基于流体的装置提供了确定测试样品中分析物的存在的精确，便宜且易于控制的方法。