公开(公告)号：US5132538A

公开(公告)日：1992-07-21

申请号：US705602

申请日：1991-05-24

Applicant: Karl Norris

Inventor： Karl Norris

IPC: G01N21/31 ,  G01N21/35

CPC classification number: G01N21/359 ,  G01N21/3563 ,  G01N21/314 ,  G01N2201/129 ,  Y10S250/91

Abstract: In a method for measuring protein content of whole grain kernels with a near infrared spectrophotometer, reflectance measurements are made at wavelengths shorter than 1600 nanometers. The wavelengths are selected to correspond with absorbance bands of the constituents of the sample. The measurements are encoded into absorbance data and are normalized by subtracting the absorbance value at a first wavelength and then dividing the resulting data by the value at a second wavelength. In accordance with one embodiment, the normalized data is substituted in a formula having coefficients determined by regression from samples having known percentages of protein in the sample to yield the percentage of protein in the unknown sample. In accordance with a second embodiment, the second differential of the absorbance data is determined and the resulting differential values are inserted in the formula having coefficients obtained from the known samples to yield the percentage or protein in the unknown sample.

Abstract translation: 在用近红外分光光度计测量全谷粒蛋白质含量的方法中，反射率测量在短于1600纳米的波长处进行。 选择波长以对应于样品的组分的吸收带。 将测量值编码为吸光度数据，并通过减去第一波长处的吸光度值然后将得到的数据除以第二波长的值来进行归一化。 根据一个实施方案，归一化数据在具有由样品中具有已知蛋白质百分比的样品的回归确定系数的式中代替以产生未知样品中蛋白质的百分比。 根据第二实施例，确定吸光度数据的第二差分，并将得到的差值插入具有从已知样品获得的系数的公式中，以产生未知样品中的百分比或蛋白质。

公开(公告)号：US4997280A

公开(公告)日：1991-03-05

申请号：US413063

申请日：1989-09-27

Applicant: Karl Norris

Inventor： Karl Norris

IPC: G01J3/02 ,  G01J3/28 ,  G01J3/433

CPC classification number: G01J3/2889 ,  G01J2003/2866 ,  G01J2003/2876 ,  G01J3/433 ,  G01N21/4738

Abstract: In a spectrophotometric instrument, a system is provided to correct for distortion caused by rapid scanning of the spectrum. In the instrument, photodetectors detect light energy which is scanned through a spectrum at a rapid rate. An amplifier amplifies the output signal generated by the photodetectors. The output signal of the amplifier is sampled at increments and the samples are converted to digital values. A first derivative is determined from the digital values by subtracting from each value the value from the preceding increment. The first derivative values are multiplied times a constant selected to correct for the distortion and the resulting product values are added to the amplitude digital values to provide a set of corrected values representing the intensity detected by the photodetectors.