METHODS TO DETECT AND QUANTIFY RNA
    1.
    发明申请
    METHODS TO DETECT AND QUANTIFY RNA 有权
    检测和定量RNA的方法

    公开(公告)号:US20120283106A1

    公开(公告)日:2012-11-08

    申请号:US13397572

    申请日:2012-02-15

    IPC分类号: C12Q1/68 C40B20/00

    摘要: Improved methods to quantitate RNA in biological or other analytical samples employ extended RNAs containing adaptors at the 5′ end and polyA sequences coupled to a tag at the 3′ end. The invention method is particularly useful in quantitating microRNAs as primers can be used that need not complement the non-conserved 3′ ends of these molecules.

    摘要翻译: 在生物或其他分析样品中定量RNA的改进方法使用在5'端含有衔接子的延伸RNA和在3'末端与标签偶联的polyA序列。 本发明方法特别可用于定量微小RNA,因为可以使用不需要补充这些分子的非保守3'末端的引物。