公开(公告)号：US10537663B2

公开(公告)日：2020-01-21

申请号：US15800436

申请日：2017-11-01

申请人： KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

发明人： Young Mee Jung ,  Soo Hyun Kim ,  Yoo Jin Seo

IPC分类号： A61L27/00 ,  A61L27/36 ,  A61L27/52 ,  A61L27/24 ,  A61L27/26

摘要： A method for preparing a decellularized tissue-based hydrogel with maximized ability of preserving various tissue-derived proteins, growth factors and cytokines by using a supercritical fluid-organic solvent system through (a) a step of decellularizing a biological tissue by bringing the same to contact with a supercritical fluid and an organic solvent at the same time; (b) a step of washing the decellularized tissue; (c) a step of preparing a decellularized tissue solution by mixing the washed decellularized tissue with one selected from an enzyme solution, an acidic solution and a mixture thereof; (d) a step of titrating the decellularized tissue solution to pH 5.5-7.8 by treating with a basic solution; and (e) a step of allowing the titrated decellularized tissue solution to stand at 30-40° C. and utilizing the same as a tissue engineering material with improved angiogenesis and tissue regeneration abilities.