公开(公告)号：US11434217B2

公开(公告)日：2022-09-06

申请号：US16958794

申请日：2018-07-23

Applicant: KOREA INSTITUTE OF OCEAN SCIENCE & TECHNOLOGY

Inventor： Joung Han Yim ,  Il-Chan Kim ,  Se Jong Han ,  Ui Joung Youn ,  Hong Kum Lee ,  Jun Hyuck Lee ,  Tai Kyoung Kim ,  Kwon Joo Yeo

IPC: C07D307/88

Abstract: The present invention can synthesize lobaric acid and four analogues thereof, which are five phenolic lichen metabolites isolated from an extract of the Antarctic lichen Stereocaulon alpinum and selectively inhibit PTP1B, by a simple, economic and efficient chemical synthesis method.

公开(公告)号：US09644194B2

公开(公告)日：2017-05-09

申请号：US14583564

申请日：2014-12-26

Applicant: KOREA INSTITUTE OF OCEAN SCIENCE & TECHNOLOGY

Inventor： Joung Han Yim ,  Bon-Hun Koo ,  Chul Soo Shin ,  Dockyu Kim ,  Il-Chan Kim ,  Se Jong Han ,  Jun Hyuck Lee ,  Ha Ju Park

IPC: C12N9/52 ,  A01N63/02 ,  C12N9/14 ,  C12N9/16 ,  A01N37/46 ,  A61K38/48 ,  A23L5/00

CPC classification number: C12N9/52 ,  A01N37/46 ,  A01N63/02 ,  A23L5/00 ,  A61K38/482 ,  C07K2299/00 ,  C12N9/14 ,  C12N9/16 ,  C12Y304/21 ,  Y02A50/473

Abstract: A cold-adapted protease derived from Pseudoalteromonas arctica PAMC 21717 or a recombinant cold-adapted protease obtained by expressing a gene encoding the cold-adapted protease in E. coli is described, and more particularly, a crystal of a protease exhibiting activity at low temperatures, a method for crystallizing the protease, a method for preparing the protease, a recombinant microorganism that expresses the protease, a method for preparing the recombinant microorganism, a method for preparing the recombinant protease and the use of the cold-adapted protease. The cold-adapted protease exhibits high activity at low temperatures, and securely maintains its enzymatic activity even in the presence of high pH, various metal ions and surfactants. Thus, it is useful in various industrial applications.

公开(公告)号：US09574185B2

公开(公告)日：2017-02-21

申请号：US14583557

申请日：2014-12-26

Applicant: KOREA INSTITUTE OF OCEAN SCIENCE & TECHNOLOGY

Inventor： Joung Han Yim ,  Bon-Hun Koo ,  Chul Soo Shin ,  Dockyu Kim ,  Il-Chan Kim ,  Se Jong Han ,  Jun Hyuck Lee ,  Ha Ju Park

IPC: C12N9/52 ,  A01N63/02 ,  C12N9/14 ,  C12N9/16 ,  A01N37/46 ,  A61K38/48

CPC classification number: C12N9/52 ,  A01N37/46 ,  A01N63/02 ,  A23L5/00 ,  A61K38/482 ,  C07K2299/00 ,  C12N9/14 ,  C12N9/16 ,  C12Y304/21 ,  Y02A50/473

Abstract: A cold-adapted protease derived from Pseudoalteromonas arctica PAMC 21717 or a recombinant cold-adapted protease obtained by expressing a gene encoding the cold-adapted protease in E. coli is described, and more particularly, a crystal of a protease exhibiting activity at low temperatures, a method for crystallizing the protease, a method for preparing the protease, a recombinant microorganism that expresses the protease, a method for preparing the recombinant microorganism, a method for preparing the recombinant protease and the use of the cold-adapted protease. The cold-adapted protease exhibits high activity at low temperatures, and securely maintains its enzymatic activity even in the presence of high pH, various metal ions and surfactants. Thus, it is useful in various industrial applications.

Abstract translation: 描述了衍生自Pseudoalteromonas arctica PAMC 21717的冷适应蛋白酶或通过在大肠杆菌中表达编码冷适应蛋白酶的基因而获得的重组冷适应蛋白酶，更具体地，描述了在低温下表现出活性的蛋白酶的晶体 蛋白酶结晶方法，蛋白酶的制备方法，表达蛋白酶的重组微生物，重组微生物的制备方法，重组蛋白酶的制备方法及冷适应蛋白酶的使用。 冷适应蛋白酶在低温下表现出高活性，即使在高pH，各种金属离子和表面活性剂的存在下也可以保持其酶活性。 因此，它在各种工业应用中是有用的。