公开(公告)号：US20170052172A1

公开(公告)日：2017-02-23

申请号：US15257338

申请日：2016-09-06

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Seung-Hyo LEE ,  Seyun KIM ,  Yong-Mahn HAN

IPC: G01N33/50 ,  C12N5/071

CPC classification number: G01N33/5067 ,  C12N5/067 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2506/02

Abstract: The present invention relates to an immune hepatotoxicity screening method using hepatocytes derived from human stem cells. After hepatocytes differentiated from human stem cells and human hepatocytes are treated with ethanol, CCl4, and acetaminophen to induce immune hepatotoxicity, a hepatocellular immunotoxic material assay system is constructed in order to verify cytokines, chemokines, and lipid mediators, which are mediators secreted from the hepatocytes, and an immunotoxic material can be confirmed in the cells having the induced hepatotoxicity by using the system. Therefore, the immune hepatotoxicity screening method using hepatocytes derived from human stem cells can be favorably used.

Abstract translation: 本发明涉及使用源自人干细胞的肝细胞的免疫肝毒性筛选方法。 肝细胞分化为人干细胞后，用乙醇，CCl4和对乙酰氨基酚处理肝细胞诱导免疫肝毒性，构建了一种肝细胞免疫毒素物质测定系统，以验证细胞因子，趋化因子和脂质介质，这些介质是从 可以通过使用该系统在具有诱导的肝毒性的细胞中确认肝细胞和免疫毒性物质。 因此，可以有利地使用使用源自人干细胞的肝细胞的免疫肝毒性筛选方法。