公开(公告)号：US20180163179A1

公开(公告)日：2018-06-14

申请号：US15123027

申请日：2015-03-17

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Won-il JEONG ,  Yong-Mahn HAN ,  Mi Young KIM ,  Hyuk Soo EUN ,  Mi-Jin JANG

IPC: C12N5/071 ,  C12Q1/32 ,  C12Q1/30 ,  C12Q1/26

CPC classification number: C12N5/0672 ,  C12N5/067 ,  C12N2500/38 ,  C12N2501/12 ,  C12Q1/02 ,  C12Q1/26 ,  C12Q1/30 ,  C12Q1/32 ,  G01N33/50

Abstract: The present invention relates to a method for improving drug metabolism function of human stem cell-derived hepatocytes. More precisely, the human stem cell-derived hepatocytes are similar in cell morphology to human hepatocytes but display reduced expressions of drug or alcohol metabolism associated enzymes and antioxidant enzymes. So, the inventors co-cultured the hepatocytes differentiated from human stem cells with mouse hepatic stellate cells. As a result, it was confirmed that alcohol mediated toxicity was reduced so that liver cell damage or apoptosis level was reduced. In the meantime, the expressions of drug and alcohol metabolism associated enzymes and antioxidant enzymes were increased.