公开(公告)号：US20230029377A1

公开(公告)日：2023-01-26

申请号：US17842610

申请日：2022-06-16

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Jin Woo Kim ,  Eun Jung Lee

IPC: C12N15/113 ,  A61P27/02 ,  C12N9/22 ,  C12N15/11

Abstract: The present invention relates to a technique capable of treating a retinal neurodegenerative disease through regeneration of a retinal nerve by targeting Prox1 in the mammalian retina using an inhibitor which inhibits Prox1 expression or migration. According to the present invention, inducing the regeneration of the damaged retina in mammals, and thus can be commonly applied to the treatment of various retinal neurodegenerative diseases causing vision loss, and furthermore, when combining with a selective retinal nerve differentiation method or the like, it is expected that the method can be used for the development of an innovative retinal regeneration method capable of selectively regenerating only specific degenerating retinal neurons.

公开(公告)号：US11851465B2

公开(公告)日：2023-12-26

申请号：US17110924

申请日：2020-12-03

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Jin Woo Kim ,  Eun Jung Lee

IPC: C07K14/47

CPC classification number: C07K14/4702

Abstract: Disclosed is a method for regulating the extracellular secretion ability of a homeoprotein and a homeoprotein variant having regulated extracellular secretion ability using the same, and more particularly, a method for increasing or decreasing the extracellular secretion ability through the increase or decrease in hydrophobicity of an amino acid sequence among homeodomain external amino acid sequences, or a homeoprotein whose extracellular secretion ability is increased or decreased through the increase or decrease in hydrophobicity of the homeodomain external amino acid sequence.

公开(公告)号：US20160250288A1

公开(公告)日：2016-09-01

申请号：US15159739

申请日：2016-05-19

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Jin Woo Kim ,  Nam-Suk Kim

IPC: A61K38/17 ,  G01N33/50

CPC classification number: A61K38/1709 ,  A61K35/30 ,  A61K48/00 ,  G01N33/5058

Abstract: The present invention relates to a pharmaceutical composition for the regeneration of nerves comprising Vax protein as an active ingredient. Vax1 is secreted in the ventral hypothalamus (vHT) explant separated from a mouse. The secreted Vax1 combines with extracellular sugar group of heparan sulfate proteoglycans (HSPGs) existing in retinal ganglion cell (RGC) axon of the retinal explant co-cultured so as for the complex invades into axonplasm. Then, the Vax1 conjugated with the sugar group activates the local protein synthesis to accelerate the growth of retinal ganglion cell axon. In some embodiments, Vax protein or a nucleic acid encoding the protein, can be used in a pharmaceutical composition for the regeneration of nerves.

Abstract translation: 本发明涉及包含Vax蛋白作为活性成分的神经再生用药物组合物。 Vax1在从小鼠分离的腹侧下丘脑（vHT）外植体中分泌。 分泌的Vax1与存在于视网膜外植体的视网膜神经节细胞（RGC）轴突中存在的硫酸乙酰肝素蛋白聚糖（HSPG）的细胞外糖组合，以使复合物侵入轴突。 然后，与糖基结合的Vax1激活局部蛋白质合成，以加速视网膜神经节细胞轴突的生长。 在一些实施方案中，Vax蛋白或编码该蛋白的核酸可用于神经再生的药物组合物中。

公开(公告)号：US12091699B2

公开(公告)日：2024-09-17

申请号：US17586234

申请日：2022-01-27

Applicant: Korea Advanced Institute of Science and Technology

Inventor： Jin Woo Kim ,  Jun Woo Park

IPC: C12P21/02

CPC classification number: C12P21/02

Abstract: A composition for enhancing the production of a homeoprotein includes an inhibitor of lysosomal function. A method for enhancing the production of a homeoprotein, includes treating cells with an inhibitor of lysosomal function, a method for producing an animal cell line continuously expressing a homeoprotein and a method for mass-producing a homeoprotein using the animal cell line.

公开(公告)号：US12091448B2

公开(公告)日：2024-09-17

申请号：US18089393

申请日：2022-12-27

Applicant: Korea Advanced Institute of Science and Technology

Inventor： Jin Woo Kim ,  Eun Jung Lee

IPC: C07K16/18 ,  C12N15/86

CPC classification number: C07K16/18 ,  C12N15/86 ,  C07K2317/24 ,  C07K2317/565 ,  C07K2317/622 ,  C12N2750/14143

Abstract: The present disclosure relates to a PROX1 (Prospero homeobox protein 1) protein binding molecule. More specifically, the binding molecule of the present disclosure relates to a binding molecule having excellent binding ability to the PROX1 protein and having an excellent neutralizing effect on the PROX1 protein, and it is very useful for diagnosis, prevention or treatment of retinal neurodegenerative diseases.

公开(公告)号：US09968651B2

公开(公告)日：2018-05-15

申请号：US15159739

申请日：2016-05-19

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Jin Woo Kim ,  Nam-Suk Kim

IPC: A61K38/17 ,  A61K48/00 ,  G01N33/50 ,  A61K35/30

CPC classification number: A61K38/1709 ,  A61K35/30 ,  A61K48/00 ,  G01N33/5058

Abstract: The present invention relates to a pharmaceutical composition for the regeneration of nerves comprising Vax protein as an active ingredient. Vax1 is secreted in the ventral hypothalamus (vHT) explant separated from a mouse. The secreted Vax1 combines with extracellular sugar group of heparan sulfate proteoglycans (HSPGs) existing in retinal ganglion cell (RGC) axon of the retinal explant co-cultured so as for the complex invades into axonplasm. Then, the Vax1 conjugated with the sugar group activates the local protein synthesis to accelerate the growth of retinal ganglion cell axon. In some embodiments, Vax protein or a nucleic acid encoding the protein, can be used in a pharmaceutical composition for the regeneration of nerves.