公开(公告)号：US20230119862A1

公开(公告)日：2023-04-20

申请号：US17907188

申请日：2021-03-19

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Hyun Gyu PARK ,  Jayeon SONG ,  Hyo Yong KIM ,  Yujin JUNG

IPC: C12Q1/6876 ,  C12Q1/686

Abstract: The present invention relates to a self-priming hairpin-mediated isothermal amplification (SP-HAMP) and, more specifically, to a hairpin probe having a self-priming structure, which is for detecting a target nucleic acid and can be used in the nucleic acid isothermal amplification, and to a method for detecting a target nucleic acid by using same. The SP-HAMP technique according to the present invention is convenient due to no need of a separate primer with a complicated design required in existing LAMP technology, has improved efficiency of detection compared with existing LAMP reactions, and can detect DNA as well as RNA as a target nucleic acid, and therefore the technique according to the present invention can be applied in a wider variety of fields.

公开(公告)号：US20210040536A1

公开(公告)日：2021-02-11

申请号：US16966839

申请日：2019-01-30

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY ,  BIONANO HEALTH GUARD RESEARCH CENTER

Inventor： Hyun Gyu PARK ,  Seoyoung LEE ,  Hyo Yong KIM ,  Hyowon JANG

IPC: C12Q1/686

Abstract: The present invention relates to an isothermal nucleic acid amplification technique based on a three-way junction structure that is formed as a ThIsAmp template; a ThIsAmp primer; and a nucleic acid are associated with each other through a hybridization reaction. Beyond the limitation of the conventional EXPAR technique, that is, the restricted application range that the EXPAR can be used only for detecting short target nucleic acids having a 3′-OH end, the method according to the present invention can detect target nucleic acids at excellent efficiency without being limited to kinds of target nucleic acids.

公开(公告)号：US20230002804A1

公开(公告)日：2023-01-05

申请号：US17929834

申请日：2022-09-06

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY ,  BIONANO HEALTH GUARD RESEARCH CENTER

Inventor： Hyun Gyu PARK ,  Hyo Yong KIM ,  Yong JU ,  Jun Ki AHN

IPC: C12Q1/683

Abstract: The present invention relates to a detection method for detecting a target RNA contained in a sample with high sensitivity by using nicking/extension chain reaction system-based isothermal nucleic acid amplification (NESBA) that uses activity of a cleavage enzyme and a DNA polymerase. The NESBA of the present invention is a new concept isothermal target RNA detection method that realizes higher amplification efficiency than the existing NASBA technology and is deemed to be utilizable as a new concept diagnosis technology that can replace conventional target RNA detection technologies.

公开(公告)号：US20200063183A1

公开(公告)日：2020-02-27

申请号：US16495435

申请日：2017-05-29

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Hyun Gyu PARK ,  Jun Ki AHN ,  Hyo Yong KIM ,  Yong JU

IPC: C12Q1/54

Abstract: The present invention relates to a method for detecting or quantifying ATP in a sample by using a blood glucose meter, the method including adding a sample to be detected to an ATP detection composition containing glucose and an enzyme involved in ATP regeneration so as to convert glucose into glucose-6-phosphate, and then measuring the glucose concentration by using a glucose meter, thereby detecting or quantifying ATP.

公开(公告)号：US20210363566A1

公开(公告)日：2021-11-25

申请号：US16958683

申请日：2018-12-31

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY ,  BIONANO HEALTH GUARD RESEARCH CENTER

Inventor： Hyun Gyu PARK ,  Hyo Yong KIM ,  Yong JU ,  Jun Ki AHN

IPC: C12Q1/683

Abstract: The present invention relates to a detection method for detecting a target RNA contained in a sample with high sensitivity by using nicking/extension chain reaction system-based isothermal nucleic acid amplification (NESBA) that uses activity of a cleavage enzyme and a DNA polymerase. The NESBA of the present invention is a new concept isothermal target RNA detection method that realizes higher amplification efficiency than the existing NASBA technology and is deemed to be utilizable as a new concept diagnosis technology that can replace conventional target RNA detection technologies.