公开(公告)号：US20210017499A1

公开(公告)日：2021-01-21

申请号：US16912973

申请日：2020-06-26

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yong JON ,  Jun Hyuk SONG ,  Daeyoup Lee ,  Yoomi Lee

IPC: C12N5/095 ,  G01N33/50 ,  C12N5/00

Abstract: The present invention relates to a method or kit for producing cancer stem cell spheroids, and a method of screening of drugs for treating cancer cell resistance using the prepared cancer stem cell spheroid, and it can conveniently produce cancer stem cell spheroids, and the prepared cancer stem cell spheroid can be effectively utilized for screening drugs for treating cancer cell resistance.

公开(公告)号：US09650633B2

公开(公告)日：2017-05-16

申请号：US14778600

申请日：2014-11-03

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Daeyoup Lee ,  Seung-Kyoon Kim ,  Hosuk Lee

IPC: C12N15/113 ,  C07K16/00 ,  C12N5/0735 ,  C07K16/18 ,  C12N15/115

CPC classification number: C12N15/113 ,  C07K16/18 ,  C07K2317/76 ,  C12N5/0606 ,  C12N15/1137 ,  C12N15/115 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/16 ,  C12N2320/30 ,  C12Y201/01043

Abstract: Provided is a composition for controlling pluripotency of stem cells including an LIN28A methylation inhibitor and a screening method of the LIN28A methylation inhibitor, and more particularly, a composition for controlling pluripotency of stem cells including an inhibitor controlling methylation of 135th lysine of LIN28A that is methylated by SET7/9, or a composition for treating cancer, and a screening method of the inhibitor, wherein the screening method includes (a) contacting a candidate material with a cell, the cell having a gene introduced thereinto; (b) measuring a methylation level of the 135th lysine of the LIN28A; and (c) selecting an inhibitor controlling methylation of the 135th lysine of the LIN28A. That is, the present invention relates to a composition for controlling pluripotency of embryonic stem cells, or an anti-cancer composition, and a screening method of the inhibitor. The method of the present invention is possible to screen materials capable of controlling pluripotency of embryonic stem cells or materials having anti-cancer activity, and the materials screened by the method of the present invention may control pluripotency of embryonic stem cells and inhibit growth of cancer cells, which is effective for disorders of stem cell differentiation or preparation of cancer therapeutic agents.

公开(公告)号：US20160053258A1

公开(公告)日：2016-02-25

申请号：US14778600

申请日：2014-11-03

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Daeyoup Lee ,  Seung-Kyoon Kim ,  Hosuk Lee

IPC: C12N15/113 ,  C12N15/115 ,  C07K16/18

CPC classification number: C12N15/113 ,  C07K16/18 ,  C07K2317/76 ,  C12N5/0606 ,  C12N15/1137 ,  C12N15/115 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/16 ,  C12N2320/30 ,  C12Y201/01043

Abstract: Provided is a composition for controlling pluripotency of stem cells including an LIN28A methylation inhibitor and a screening method of the LIN28A methylation inhibitor, and more particularly, a composition for controlling pluripotency of stem cells including an inhibitor controlling methylation of 135th lysine of LIN28A that is methylated by SET7/9, or a composition for treating cancer, and a screening method of the inhibitor, wherein the screening method includes (a) contacting a candidate material with a cell, the cell having a gene introduced thereinto; (b) measuring a methylation level of the 135th lysine of the LIN28A; and (c) selecting an inhibitor controlling methylation of the 135th lysine of the LIN28A. That is, the present invention relates to a composition for controlling pluripotency of embryonic stem cells, or an anti-cancer composition, and a screening method of the inhibitor. The method of the present invention is possible to screen materials capable of controlling pluripotency of embryonic stem cells or materials having anti-cancer activity, and the materials screened by the method of the present invention may control pluripotency of embryonic stem cells and inhibit growth of cancer cells, which is effective for disorders of stem cell differentiation or preparation of cancer therapeutic agents.

Abstract translation: 提供了用于控制包括LIN28A甲基化抑制剂和LIN28A甲基化抑制剂的筛选方法的干细胞的多能性的组合物，更具体地，涉及用于控制干细胞多能性的组合物，其包括控制甲基化的LIN28A的第135位赖氨酸甲基化的抑制剂 通过SET7 / 9或用于治疗癌症的组合物和抑制剂的筛选方法，其中所述筛选方法包括（a）使候选物质与细胞接触，所述细胞具有引入其中的基因; （b）测量LIN28A的第135位赖氨酸的甲基化水平; 和（c）选择控制LIN28A的第135位赖氨酸甲基化的抑制剂。 也就是说，本发明涉及用于控制胚胎干细胞的多能性的组合物，或抗癌组合物，以及抑制剂的筛选方法。 本发明的方法可以筛选能够控制胚胎干细胞的多能性或具有抗癌活性的材料，并且通过本发明的方法筛选的材料可以控制胚胎干细胞的多能性并抑制癌症的生长 细胞，其对于干细胞分化障碍或癌症治疗剂的制备是有效的。