公开(公告)号：US07335471B2

公开(公告)日：2008-02-26

申请号：US10472146

申请日：2002-03-19

Applicant: Jean Guillerez ,  Pascal Lopez ,  Marc Dreyfus

Inventor： Jean Guillerez ,  Pascal Lopez ,  Marc Dreyfus

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12N9/1247 ,  C12P21/00

Abstract: Mutant RNA polymerases of phagic origin in which the peptide chain is modified by substitution, deletion or addition of at least one amino acid, the modification having the effect of reducing the sensitivity of the RNA polymerases to the initial transcription sequence of the DNA sequence coding for the RNA, for a method for production of the RNA, or proteins coded by the RNA, from given nucleotide sequences, comprising a sequence of DNA coding for the RNA, the transcription of which is placed under the control of a promoter recognised by wild-type RNA polymerases and the mutant RNA polymerases as above. The method has a higher yield of RNA than the yield obtained when using the wild-type RNA polymerases in the presence of the same non-consensual ITS as that found in the sequence of DNA coding for the RNA.

Abstract translation: 通过取代，缺失或添加至少一个氨基酸来修饰肽链的突变型RNA聚合酶，所述修饰具有降低RNA聚合酶对于编码DNA聚合酶的DNA序列的初始转录序列的敏感性的作用 RNA，用于生产RNA的方法或由RNA编码的蛋白质，从给定的核苷酸序列，包括编码RNA的DNA序列，其转录置于由野生型识别的启动子的控制下， RNA聚合酶和突变型RNA聚合酶。 该方法在使用野生型RNA聚合酶时在与编码RNA的DNA序列相同的非自愿ITS存在下获得的产量高于RNA产量。