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公开(公告)号：US20240191215A1
公开(公告)日：2024-06-13
申请号：US18287742
申请日：2022-04-20
CPC分类号： C12N9/1205 , C12Q1/48 , C12Y207/01095
摘要： Described herein are non-naturally occurring neomycin phosphotransferase (NPT) proteins and nucleic acid sequences encoding such NPT proteins. In a specific embodiment, the non-naturally occurring NPT proteins have reduced activity relative to wild-type NPT. The non-naturally occurring NPT proteins provided herein are useful as a selectable marker for screening transformed or transfected cells. Also provided herein are vectors and kits comprising a nucleic acid sequence encoding a non-naturally occurring NPT protein, and methods of producing cells expressing the non-naturally occurring NPT protein and a protein of interest or a non-coding RNA sequence of interest.
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公开(公告)号：US20210032657A1
公开(公告)日：2021-02-04
申请号：US16934970
申请日：2020-07-21
摘要： Recombinant constructs, cells and means for improved production of Adeno-Associated Viruses (AAVs) are described. Also described are methods of using the constructs and cells to produce recombinant AAVs.
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公开(公告)号：US12049638B2
公开(公告)日：2024-07-30
申请号：US16934970
申请日：2020-07-21
CPC分类号： C12N15/86 , C12N7/00 , C12N2750/14121 , C12N2750/14151 , C12N2800/50 , C12N2830/36 , C12N2830/40 , C12N2830/42 , C12N2830/50
摘要： Disclosed are non-naturally occurring nucleic acid molecules comprising nucleotide sequences encoding serine recombinases. Also disclosed are vectors comprising non-naturally occurring nucleic acid molecule, and cells comprising the non-naturally occurring nucleic acid molecule or the vector. Recombinant constructs, cells and means for improved production of Adeno-Associated Viruses (AAVs) are described. Also described are methods of using the constructs and cells to produce recombinant AAVs.
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