公开(公告)号：US20240402141A1

公开(公告)日：2024-12-05

申请号：US18697074

申请日：2022-09-09

Applicant: JSR CORPORATION

Inventor： Minato AKIYAMA ,  Kunihiko KOBAYASHI ,  Yukiya INOUE ,  Masahiro KIKUCHI

IPC: G01N30/52 ,  G01N33/68

Abstract: Provided is a method for filling a column with a chromatography carrier, including substituting a slurry with an aqueous solvent not containing a buffer, in which the slurry contains a target substance-capturing chromatography carrier, a buffer having an acid dissociation constant (pKa) within a range of ±1.0 of the isoelectric point of the carrier, and an aqueous solvent and has a pH of a liquid phase adjusted within a range of ±2.0 of the isoelectric point of the carrier, and filling a column with the slurry subjected to solvent substitution. The method may be advantageous for suppressing a decrease in liquid permeability and pressure resistance characteristics during liquid passage of a chromatography carrier when a solvent is substituted with an aqueous solvent not containing a buffer.

公开(公告)号：US20240116027A1

公开(公告)日：2024-04-11

申请号：US18551755

申请日：2022-03-15

Applicant: JSR CORPORATION

Inventor： Kunihiko KOBAYASHI ,  Minato AKIYAMA ,  Yukiya INOUE ,  Masahiro KIKUCHI

IPC: B01J20/285 ,  B01D15/20 ,  B01D15/22 ,  B01J20/30

CPC classification number: B01J20/285 ,  B01D15/206 ,  B01D15/22 ,  B01J20/3092

Abstract: A chromatography carrier may exhibit high liquid permeability and an excellent pressure-resistant characteristic during liquid passage. A chromatography carrier production method may include: (1) providing a solid phase support, the solid phase support being formed of porous particles on which a ligand has or has not been immobilized; and (2) subjecting the solid phase support to sieve classification, the coefficient of variation of the volume particle size distribution of the porous particles when a ligand has been immobilized being adjusted to 1% to 22%. The skewness of the volume particle size distribution of the porous particles when a ligand has been immobilized may be adjusted to −0.1 to 5.

公开(公告)号：US20240165588A1

公开(公告)日：2024-05-23

申请号：US18551116

申请日：2022-03-15

Applicant: JSR CORPORATION

Inventor： Kunihiko KOBAYASHI ,  Minato AKIYAMA ,  Yukiya INOUE ,  Masahiro KIKUCHI

IPC: B01J20/286 ,  B01D15/20 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/30 ,  B01J20/32

CPC classification number: B01J20/286 ,  B01D15/206 ,  B01D15/3828 ,  B01J20/265 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/3092 ,  B01J20/321 ,  B01J20/3217 ,  B01J20/3278 ,  B01J20/3293 ,  B01J2220/52

Abstract: A method for producing a chromatography carrier, including providing a solid phase support, where the solid phase support provided is formed of porous particles on which a ligand has or has not been immobilized, and subjecting the solid phase support to sieve classification. A coefficient of variation of a volume particle size distribution of the porous particles when a ligand has been immobilized is adjusted to 1% to 22%, and a ratio (d1/d50) of volume cumulative 1% particle size d1 to volume cumulative 50% particle size d50 in terms of the porous particles is adjusted to 0.55 to 1.0.