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公开(公告)号：US12261013B2
公开(公告)日：2025-03-25
申请号：US17964516
申请日：2022-10-12
Applicant: JEOL Ltd.
Inventor： Takeshi Kaneko , Isamu Ishikawa , Eiji Okunishi
Abstract: Provided is a charged particle beam system capable of preventing the data acquisition time from increasing. A control method for the system is also provided. The charged particle beam system includes: a beam blanker for blanking a charged particle beam; a sample stage on which a sample is tiltably held and thus can assume a tilt angle; a blanking controller for controlling the blanking of the charged particle beam and causing a pulsed beam having a duty ratio to be directed at the sample; and a tilt controller for controlling the tilt angle of the sample. The blanking controller sets the duty ratio of the pulsed beam based on the tilt angle of the sample.
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公开(公告)号：US10541107B2
公开(公告)日：2020-01-21
申请号：US15505462
申请日：2015-08-21
Inventor： Yoshinori Fujiyoshi , Isamu Ishikawa , Naoki Hosogi
Abstract: A three-dimensional image reconstruction method associated with the present invention includes the steps of: obtaining a first transmission electron microscope image of a sample containing the membrane proteins present within a lipid membrane, the image having been taken by illuminating an electron beam on the sample from a direction tilted relative to a line normal to the membrane surface of the lipid membrane; obtaining a second transmission electron microscope image of the sample taken by illuminating the electron beam on the sample perpendicularly to the membrane surface of the lipid membrane; identifying orientations of the membrane proteins of the first transmission electron microscope image on a basis of the second transmission electron microscope image; and analyzing a three-dimensional structure of the membrane proteins from the first transmission electron microscope image on a basis of information about the identified orientations of the membrane proteins.
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公开(公告)号：US20230115486A1
公开(公告)日：2023-04-13
申请号：US17964516
申请日：2022-10-12
Applicant: JEOL Ltd.
Inventor： Takeshi Kaneko , Isamu Ishikawa , Eiji Okunishi
Abstract: Provided is a charged particle beam system capable of preventing the data acquisition time from increasing. A control method for the system is also provided. The charged particle beam system includes: a beam blanker for blanking a charged particle beam; a sample stage on which a sample is tiltably held and thus can assume a tilt angle; a blanking controller for controlling the blanking of the charged particle beam and causing a pulsed beam having a duty ratio to be directed at the sample; and a tilt controller for controlling the tilt angle of the sample. The blanking controller sets the duty ratio of the pulsed beam based on the tilt angle of the sample.
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4.发明申请公开(公告)号：US20170278669A1
公开(公告)日：2017-09-28
申请号：US15505462
申请日：2015-08-21
Inventor： Yoshinori Fujiyoshi , Isamu Ishikawa , Naoki Hosogi
Abstract: A three-dimensional image reconstruction method associated with the present invention comprises the steps of: obtaining a first transmission electron microscope image of a sample containing the membrane proteins present within a lipid membrane, the image having been taken by illuminating an electron beam on the sample from a direction tilted relative to a line normal to the membrane surface of the lipid membrane (step S10); obtaining a second transmission electron microscope image of the sample taken by illuminating the electron beam on the sample perpendicularly to the membrane surface of the lipid membrane (step S12); identifying orientations of the membrane proteins of the first transmission electron microscope image on a basis of the second transmission electron microscope image (step S14); and analyzing a three-dimensional structure of the membrane proteins from the first transmission electron microscope image on a basis of information about the identified orientations of the membrane proteins (step S18).
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