公开(公告)号：US20190012520A1

公开(公告)日：2019-01-10

申请号：US16066879

申请日：2016-11-07

Applicant: Institute of Automation, Chinese Academy of Sciences

Inventor： Guoqing LI ,  Hongtu MA ,  Hua HAN ,  Lixin WEI

IPC: G06K9/00 ,  G06K9/62 ,  G06K9/68

CPC classification number: G06K9/00134 ,  G06K9/0014 ,  G06K9/00147 ,  G06K9/6206 ,  G06K9/6211 ,  G06K9/6257 ,  G06K9/6262 ,  G06K9/6269 ,  G06K9/6857

Abstract: A method for microscopic image acquisition based on a sequential slice. The method includes; acquiring a sample of the sequential slice and a navigation image thereof; identifying and labeling the sample of the sequential slice in the navigation image by utilizing methods of image processing and machine learning; placing the sample of the sequential slice in a microscope, establishing a coordinate transformation matrix for a navigation image-microscope actual sampling space coordinate, and navigating and locating a random pixel point in the navigation image to a center of the microscope's visual field; locating the sample of the sequential slice under a low resolution visual field, binding a sample acquisition parameter; based on the binding of the sample acquisition parameter, recording a relationship of relative of locations between a center point of a high resolution acquisition region and a center point after being matched with a sample template.