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1.发明申请METHODS OF PROCESSING SUGAR CANE AND SWEET SORGHUM WITH INTEGRATED CONVERSION OF PRIMARY AND LIGNOCELLULOSIC SUGARS 审中-公开加工粗糖和甜菜糖的方法与主要和肉桂糖的综合转化公开(公告)号：US20160355858A1
公开(公告)日：2016-12-08
申请号：US15117969
申请日：2015-02-11
CPC分类号： C12P19/14 , C07C31/08 , C10L5/445 , C10L2290/24 , C10L2290/26 , C10L2290/544 , C10L2290/547 , C12P7/10 , C12P7/14 , C12P19/02 , C12P2201/00 , C12Y302/01004 , Y02E50/10 , Y02E50/16 , Y02E50/30
摘要： The current invention concerns methods and products related to the production of alcohol from sugar cane and/or sweet sorghum with integration of 1st and 2nd generation (1G/2G) biorefining, thus comprising the integrated conversion of primary and secondary soft lignocellulosic biomass. In particular, methods of processing sugar cane and/or sweet sorghum feedstock are disclosed, said methods comprising the steps of providing raw juice from the feedstock, recovering a residual bagasse, pretreating the bagasse and mixing it with some quantity of raw juice, and hydrolyzing the pretreated bagasse enzymatically.
摘要翻译： 本发明涉及与通过第一代和第二代（1G / 2G）生物精炼相结合的甘蔗和/或甜高粱生产醇的方法和产品，从而包括初级和次级软木质纤维素生物质的综合转化。 特别地，公开了处理甘蔗和/或甜高粱原料的方法，所述方法包括以下步骤：从原料中提供原汁，回收残余的甘蔗渣，预处理甘蔗渣并将其与一定量的原汁混合并水解 预处理的甘蔗渣酶促。
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2.发明申请METHODS OF PROCESSING LIGNOCELLULOSIC BIOMASS USING SINGLE-STAGE AUTOHYDROLYSIS AND ENZYMATIC HYDROLYSIS WITH C5 BYPASS AND POST-HYDROLYSIS 审中-公开使用单阶段自动分解和酶解水解方法对C5旁路和后水解进行处理生物质的方法公开(公告)号：US20150191758A1
公开(公告)日：2015-07-09
申请号：US14418667
申请日：2013-08-01
申请人： Inbicon A/S
CPC分类号： C12P19/14 , C12P7/10 , C12P19/02 , C12P2201/00 , C13K1/02 , C13K13/002 , D21C1/04 , D21C3/04 , Y02E50/16
摘要： The invention relates, in general, to methods of processing lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft lignocellulosic biomass feedstocks by pretreating to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subject to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.
摘要翻译： 本发明一般涉及将木质纤维素生物质加工成可发酵糖的方法以及依赖于水热预处理的方法。 可以通过在单级加压水热预处理中预处理至非常低的严重性，然后进行酶水解以释放保持在固体状态的木糖，从而可以从软木质纤维素生物质原料获得与使用两阶段预处理的那些相当的木糖单体产率。 在一些实施方案中，预处理的生物质被分离成固体级分和液体级分，固体级分进行酶水解，并且分离的液体级分随后与水解的固体级分混合。
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公开(公告)号：US20240102064A1
公开(公告)日：2024-03-28
申请号：US18523358
申请日：2023-11-29
申请人： Inbicon A/S
CPC分类号： C12P19/14 , C12P7/10 , C12P19/02 , C13K1/02 , C13K13/002 , D21C1/04 , D21C3/04 , C12P2201/00 , Y02E50/10
摘要： The invention relates, in general, to methods of processing Lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft Lignocellulosic biomass feedstocks by pretreasting to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.
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公开(公告)号：US20210348202A1
公开(公告)日：2021-11-11
申请号：US17329960
申请日：2021-05-25
申请人： Inbicon A/S
摘要： The invention relates, in general, to methods of processing Lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft Lignocellulosic biomass feedstocks by pretreasting to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.
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公开(公告)号：US11118203B2
公开(公告)日：2021-09-14
申请号：US14418667
申请日：2013-08-01
申请人： Inbicon A/S
摘要： The invention relates, in general, to methods of processing lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft lignocellulosic biomass feedstocks by pretreating to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.
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公开(公告)号：US20190271017A1
公开(公告)日：2019-09-05
申请号：US16347091
申请日：2017-11-06
申请人： Inbicon A/S
发明人： Martin Dan Jeppesen , Jan Larsen , Kit Haubjerg Mogensen , Anna Frederike Gossmann , Anna Granly Hansen , Laila Thirup , Lars Villadsgaard Toft , Hanne Risbjerg Sørensen
摘要： A method for providing a C5/C6 product from a lignocellulosic material is disclosed, said method comprising the steps: (i) pretreatment of the lignocellulosic material; (ii) solid/liquid separation of the pretreated lignocellulosic material from step (a) into a first solid fraction and a first liquid fraction; (iii) enzymatic fiber hydrolysis of said first solid fraction from step (b) by use of an enzyme composition capable of degrading lignocellulosic material, thereby providing a C5/C6 fiber slurry comprising C5 and/or C6 sugars; (iv) solid/liquid separation of the C5/C6 fiber slurry from step (c) into a second solid fraction and a second liquid fraction; and optionally (v) combining said first liquid fraction and said second liquid fraction for enzymatic mixed sugar hydrolysis (MSH), whereby a MSH C5/C6 product is provided.
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公开(公告)号：US11866753B2
公开(公告)日：2024-01-09
申请号：US17329960
申请日：2021-05-25
申请人： Inbicon A/S
CPC分类号： C12P19/14 , C12P7/10 , C12P19/02 , C13K1/02 , C13K13/002 , D21C1/04 , D21C3/04 , C12P2201/00 , Y02E50/10
摘要： The invention relates, in general, to methods of processing Lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft Lignocellulosic biomass feedstocks by pretreasting to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.
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公开(公告)号：US09920345B2
公开(公告)日：2018-03-20
申请号：US14908913
申请日：2014-02-05
申请人： Inbicon A/S
CPC分类号： C12P19/14 , C12P5/023 , C12P7/10 , C12P19/02 , C12P2201/00 , D21C1/02 , D21C1/04 , D21C5/005 , Y02E50/16 , Y02E50/343
摘要： Methods of processing lignocellulosic biomass to fermentable sugars are provided which rely on hydrothermal pretreatment. Soft lignocellulosic biomass feedstock is pretreated in a single-stage pressurized hydrothermal pretreatment to very low severity. The pre-treated biomass is hydrolysed, typically as a whole slurry, using enzymatic hydrolysis catalysed by an enzyme mixture comprising endoglucanase, exoglucanase, β-glucosidase, endoxylanase, and β-xylosidase activities at activity levels in nkat/g glucan of endoglucanase of at least 1100, exoglucanase of at least 280, β-glucosidase of at least 3000, endoxylanase of at least 1400, and β-xylosidase of at least 75, so as to produce a hydrolysate in which the yield of C5 monomers is at least 55% of the original xylose and arabinose content of the feedstock prior to pretreatment.
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9.发明申请METHODS OF PROCESSING LIGNOCELLULOSIC BIOMASS USING SINGLE-STAGE AUTOHYDROLYSIS PRETREATMENT AND ENZYMATIC HYDROLYSIS 有权使用单级自动溶解预处理和酶水解处理线粒体生物量的方法公开(公告)号：US20160160253A1
公开(公告)日：2016-06-09
申请号：US14908913
申请日：2014-02-05
申请人： INBICON A/S
CPC分类号： C12P19/14 , C12P5/023 , C12P7/10 , C12P19/02 , C12P2201/00 , D21C1/02 , D21C1/04 , D21C5/005 , Y02E50/16 , Y02E50/343
摘要： Methods of processing lignocellulosic biomass to fermentable sugars are provided which rely on hydrothermal pretreatment. Soft lignocellulosic biomass feedstock is pretreated in a single-stage pressurized hydrothermal pretreatment to very low severity. The pre-treated biomass is hydrolysed, typically as a whole slurry, using enzymatic hydrolysis catalysed by an enzyme mixture comprising endoglucanase, exoglucanase, β-glucosidase, endoxylanase, and β-xylosidase activities at activity levels in nkat/g glucan of endoglucanase of at least 1100, exoglucanase of at least 280, β-glucosidase of at least 3000, endoxylanase of at least 1400, and β-xylosidase of at least 75, so as to produce a hydrolysate in which the yield of C5 monomers is at least 55% of the original xylose and arabinose content of the feedstock prior to pretreatment.
摘要翻译： 提供了依靠水热预处理将木质纤维素生物质加工成可发酵糖的方法。 软木质纤维素生物质原料在单级加压水热预处理中预处理至非常低的严重性。 使用由含有内切葡聚糖酶，外切葡聚糖酶，葡萄糖苷酶，内切木聚糖酶和β-木糖苷酶活性的酶混合物催化的酶水解，预处理的生物质通常作为整体浆液水解，其活性水平在内切葡聚糖酶的nkat / g葡聚糖 至少为1100，至少280的外切葡聚糖酶，至少为3000的葡萄糖苷酶，至少为1400的内切木聚糖酶，至少为75的木糖苷酶，以及至少75的β-木糖苷酶，以产生其中C5单体的产率为 在预处理之前，原料中原始木糖和阿拉伯糖含量的至少55％。
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