公开(公告)号：US10954545B2

公开(公告)日：2021-03-23

申请号：US15565175

申请日：2016-04-11

申请人： Icosagen Cell Factory OÜ

发明人： Mart Ustav ,  Ene Ustav ,  Andres Männik ,  Mart Toots ,  Mart Ustav, Jr. ,  Andres Tover

IPC分类号： C12N15/11 ,  A61K38/00 ,  A61K48/00 ,  C07K14/705 ,  C12N15/86 ,  C12Q1/18 ,  C12Q1/70 ,  C12N5/09

摘要： A U2OS-based model system using luciferase reporters to monitor the genome replication of alpha and beta HPVs is provided. A modified U2OS cell line is disclosed that expresses Firefly luciferase to measure toxicity of the screened compounds. In addition, provided are HPV18, HPV 16 and HPV5 marker genomes that express Renilla luciferase under the control of viral promoters used to measure changes in the viral copy number. This ready-to-use model system is capable of being used in high-throughput screens to identify compounds inhibiting initial amplification and stable maintenance as well as vegetative phase of various HPV subtypes.

公开(公告)号：US11000529B2

公开(公告)日：2021-05-11

申请号：US16568310

申请日：2019-09-12

申请人： Icosagen Cell Factory OÜ

发明人： Mart Ustav, Sr. ,  Ene Ustav ,  Andres Männik ,  Mart Toots ,  Andres Tover

IPC分类号： A61K31/566 ,  A61K31/52 ,  A61K31/568 ,  A61K31/341 ,  A61K31/4184 ,  A61K31/517 ,  A61K31/496 ,  A61K31/57 ,  A61K31/575 ,  A61P31/20 ,  A61K31/58

摘要： Methods for treating high risk HPV infection by inhibiting one or more phases of HPV replication cycle with antiviral compounds are disclosed. Moreover, a mechanism for the inhibition is suggested and targets for further antiviral compounds are disclosed.

公开(公告)号：US10543218B2

公开(公告)日：2020-01-28

申请号：US15573362

申请日：2016-05-11

申请人： Icosagen Cell Factory OÜ

发明人： Mart Ustav, Sr. ,  Ene Ustav ,  Andres Männik ,  Mart Toots ,  Andres Tover

IPC分类号： A61K31/4184 ,  A61K31/496 ,  A61K31/341 ,  A61K31/566 ,  A61K31/517 ,  A61K31/52 ,  A61P31/20 ,  A61K31/568 ,  A61K31/58

摘要： Novel antiviral compounds for inhibiting one or more phases of HPV replication cycle are disclosed. Moreover, a mechanism for the inhibition is suggested and targets for further antiviral compounds are disclosed.

公开(公告)号：US20180133228A1

公开(公告)日：2018-05-17

申请号：US15573362

申请日：2016-05-11

申请人： Icosagen Cell Factory OÜ

发明人： Mart Ustav, Sr. ,  Ene Ustav ,  Andres Männik ,  Mart Toots ,  Andres Tover

IPC分类号： A61K31/566 ,  A61P31/20 ,  A61K31/52 ,  A61K31/568 ,  A61K31/58 ,  A61K31/341 ,  A61K31/4184 ,  A61K31/517

摘要： Novel antiviral compounds for inhibiting one or more phases of HPV replication cycle are disclosed. Moreover, a mechanism for the inhibition is suggested and targets for further antiviral compounds are disclosed.