公开(公告)号：US11236343B2

公开(公告)日：2022-02-01

申请号：US16346251

申请日：2017-11-01

Applicant: INSTITUTE FOR BASIC SCIENCE ,  POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Won Jong Kim ,  Jeong Hun Kim ,  Jihyun Lee ,  Byung Joo Lee

IPC: C12N15/115 ,  A61K47/61 ,  A61P27/02 ,  A61P35/00 ,  A61K31/713

Abstract: The present invention relates to a method for producing dextran polymer-based amplified nucleic acid aptamer nanoconstructs which efficiently and selectively capture a specific target molecule, the method comprising linking a short nucleic acid sequence or a complementary sequence for formation of nanoconstructs and a nucleic acid aptamer sequence for capture of the specific target molecule to a dextran polymer by a chemical reaction, mixing the resulting polymer/nucleic acid substances with each other to form nanostructures, subjecting the nanostructures to rolling circle amplification, thereby forming a nucleic acid aptamer having a repeated structure. The dextran polymer-based amplified nucleic acid aptamer nanoconstructs have the effect of efficiently and selectively capturing a specific target molecule.