公开(公告)号：US11352666B2

公开(公告)日：2022-06-07

申请号：US15526528

申请日：2015-11-13

Applicant: INSTITUTE FOR BASIC SCIENCE

Inventor： Jin Soo Kim ,  Dae Sik Kim ,  Sang Su Bae

IPC: C12Q1/6874 ,  C12N9/22 ,  C12Q1/686 ,  C12Q1/6806 ,  G16B30/00 ,  G16B30/10

Abstract: The present disclosure relates to a method for detecting off-target sites of a programmable nuclease in a genome, and specifically, to a method for detecting off-target sites through data analysis by subjecting the genome isolated in vitro to programmable nucleases to cleave the genome and then performing whole genome sequencing or deep sequencing, and to a method for selecting on-target sites of a programmable nuclease, which minimizes the off-target effect, using this method. The Digenome-seq of the present disclosure can detect the off-target sites of a programmable nuclease on the genomic scale at a high degree of reproducibility, and thus can be used in the manufacture of programmable nucleases having high target specificity and the study thereof.

公开(公告)号：US20190153530A1

公开(公告)日：2019-05-23

申请号：US15526528

申请日：2015-11-13

Applicant: INSTITUTE FOR BASIC SCIENCE

Inventor： Jin Soo Kim ,  Dae Sik Kim ,  Sang Su Bae

IPC: C12Q1/6874 ,  C12N9/22 ,  C12Q1/6806 ,  C12Q1/686

Abstract: The present disclosure relates to a method for detecting off-target sites of a programmable nuclease in a genome, and specifically, to a method for detecting off-target sites through data analysis by subjecting the genome isolated in vitro to programmable nucleases to cleave the genome and then performing whole genome sequencing or deep sequencing, and to a method for selecting on-target sites of a programmable nuclease, which minimizes the off-target effect, using this method. The Digenome-seq of the present disclosure can detect the off-target sites of a programmable nuclease on the genomic scale at a high degree of reproducibility, and thus can be used in the manufacture of programmable nucleases having high target specificity and the study thereof.