公开(公告)号：US20040191768A1

公开(公告)日：2004-09-30

申请号：US10822711

申请日：2004-04-13

申请人： INNOGENETICS, S.A.

发明人： Geert Maertens ,  Lieven Stuyver ,  Rudi Rossau ,  Hugo Van Heuverswyn

IPC分类号： C12Q001/70

CPC分类号： C12Q1/707 ,  C12Q1/6834 ,  C12Q2563/131 ,  C12Q2545/101 ,  C12Q2525/101 ,  C12Q2531/113

摘要： The invention relates to a process for genotyping any HCV isolate present in a biological sample, previously identified as being HCV positive, and for classifying said isolate according to the percentage of homology with other HCV isolates, comprising the steps of: contacting said sample in which the ribonucleotides or deoxyribonucleotides have been made accessible, if need be, under suitable denaturation, with at least one probe from about 10 to about 40 nucleotides, with said probe being liable to hybridize to a region being in the domain extending from nucleotide at position null291 to nucleotide at position null66 of the 5null untranslated region of one of the HCV isolates represented by their cDNA sequences, with said numbering of position beginning with the first ATG codon of the open reading frame encoding the HCV polyprotein, or with said probe being complementary to the above-defined probes, detecting the complexes possibly formed between said probe and the nucleotide sequence of the HCV isolate to be identified.

摘要翻译： 本发明涉及用于对先前鉴定为HCV阳性的生物样品中存在的任何HCV分离物进行基因分型的方法，以及根据与其它HCV分离株的同源性百分比对所述分离物进行分类的方法，包括以下步骤： 如果需要的话，核糖核苷酸或脱氧核糖核苷酸可以在合适的变性下与至少一个约10至约40个核苷酸的探针接触，所述探针易于与位置延伸的结构域延伸的区域杂交。 291到由其cDNA序列表示的HCV分离物之一的5'非翻译区的-66位的核苷酸，其中所述编号的位置开始于编码HCV多蛋白的开放阅读框的第一个ATG密码子，或与所述探针 与上述定义的探针互补，检测可能形成在所述探针与H的核苷酸序列之间的复合物 CV分离鉴定。

公开(公告)号：US20040126754A1

公开(公告)日：2004-07-01

申请号：US10685435

申请日：2003-10-16

申请人： N.V. INNOGENETICS S.A.

发明人： Geert Maertens ,  Erik Depla

IPC分类号： C12Q001/70 ,  C07K016/08 ,  C07K014/02

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2770/24222 ,  G01N33/56983 ,  G01N2500/00

摘要： Multimer peptides (e.g. 30- to 45-mer peptides) derived from hepatitis C virus envelope proteins reacting with the majority of anti-HCV antibodies present in patient sera are described. The usage of the latter peptides to diagnose, and to vaccinate against, an infection with hepatitis C virus is also disclosed.

摘要翻译： 描述了源自患有血清中存在的大多数抗HCV抗体的丙型肝炎病毒包膜蛋白的多聚体肽（例如30-45聚体肽）。 还公开了后一种肽用于丙型肝炎病毒感染的诊断和疫苗接种。

公开(公告)号：US20030064360A1

公开(公告)日：2003-04-03

申请号：US09873224

申请日：2001-06-05

申请人： N.V. INNOGENETICS S.A.

发明人： Geert Maertens ,  Lieven Stuyver

IPC分类号： C12Q001/70 ,  C07H021/04 ,  C12N007/00 ,  C12P021/02 ,  C12N005/06 ,  C12N015/86

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C12N2770/24222

摘要： The present invention relates to a polynucleic acid composition comprising or consisting of at least one polynucleic acid containing 8 or more contiguous nucleotides corresponding to a nucleotide sequence from the region spanning positions 417 to 957 of the Core/E1 region of HCV type 3; and/or the region spanning positions 4664 to 4730 of the NS3 region of HCV type 3: and/or the region spanning positions 4892 to 5292 of the NS3/4 region of HCV type 3; and/or the region spacing positions 8023 to 8235 of the NS5 region of the BR36 subgroup of HCV type 3a; and/or the coding region of HCV type 4a starting at nucleotide 379 in the core region; and/or the coding region of HCV type 4; and/or the coding region of HCV type 5, with said nucleotide numbering being with respect to the numbering of HCV nucleic acids as shown in Table 1, and with said polynucleic acids containing at least one nucleotide difference with known HCV type 1, and/or HCV type 2 genomes in the above-indicated regions, or the complement thereof.

摘要翻译： 本发明涉及包含至少一种含有8个或更多个连续核苷酸的多核苷酸的多核酸组合物，其对应于来自HCV 3型核心/ E1区域的位置417至957的区域的核苷酸序列; 和/或跨越HCV 3型NS3区域的位置4664至4730的区域和/或跨越HCV 3型NS3 / 4区域的位置4892至5292的区域; 和/或HCV3型BR36亚组的NS5区域的区域间隔位置8023至8235; 和/或从核心区域的核苷酸379开始的HCV型4a的编码区; 和/或HCV 4型的编码区; 和/或HCV 5型的编码区，其中所述核苷酸编号相对于表1所示的HCV核酸的编号，并且所述多核苷酸与已知的HCV I型具有至少一个核苷酸差异，和/ 或上述区域中的HCV 2型HCV基因组，或其补体。

公开(公告)号：US20030008274A1

公开(公告)日：2003-01-09

申请号：US09899046

申请日：2001-07-06

申请人： N.V. Innogenetics S.A.

发明人： Geert Maertens ,  Lieven Stuyver

IPC分类号： C12Q001/70 ,  C07H021/04 ,  A61K039/29 ,  A61K048/00

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C12N2770/24222 ,  Y02A50/396

摘要： The present invention relates to a polynucleic acid composition comprising or consisting of at least one polynucleic acid containing 8 or more contiguous nucleotides corresponding to a nucleotide sequence from the region spanning positions 417 to 957 of the Core/E1 region of HCV type 3; and/or the region spanning positions 4664 to 4730 of the NS3 region of HCV type 3; and/or the region spanning positions 4892 to 5292 of the NS3/4 region of HCV type 3; and/or the region spanning positions 8 023 to 8 235 of the NS5 region of the BR36 subgroup of HCV type 3a and/or the coding region of HCV type 4a starting at nucleotide 379 in the core region; and/or the coding region of HCV type 4; and/or the coding region of HCV type 5, with said nucleotide numbering being with respect to the numbering of HCV nucleic acids as shown in Table 1, and with said polynucleic acids containing at least one nucleotide difference with known HCV type 1, and/or HCV type 2 genomes in the above-indicated regions, or the complement thereof.

摘要翻译： 本发明涉及包含至少一种含有8个或更多个连续核苷酸的多核苷酸的多核酸组合物，其对应于来自HCV 3型核心/ E1区域的位置417至957的区域的核苷酸序列; 和/或跨越HCV 3型NS3区域的位置4664至4730的区域; 和/或跨越HCV 3型NS3 / 4区的位置4892至5292的区域; 和/或从核心区域的核苷酸379开始的HCV 3A型BR36亚组的NS5区域和/或HCV型4a编码区的位置8 023至8 235的区域; 和/或HCV 4型的编码区; 和/或HCV 5型的编码区，其中所述核苷酸编号相对于表1所示的HCV核酸的编号，并且所述多核苷酸与已知的HCV I型具有至少一个核苷酸差异，和/ 或上述区域中的HCV 2型HCV基因组，或其补体。