公开(公告)号：US20210257045A1

公开(公告)日：2021-08-19

申请号：US17186816

申请日：2021-02-26

Applicant: Hoffmann-La Roche Inc.

Inventor： Tobias GROSSKOPF ,  Oliver POPP ,  Tobias WALLOCHA

IPC: G16B5/00 ,  G16B50/10

Abstract: Herein is reported a method for determining if process data acquired during the cultivation of a mammalian or bacterial cell is affected by a problem comprising the steps of (i) fitting the process data acquired during the cultivation of a mammalian or bacterial cell clone expressing a recombinant, heterologous polypeptide in a metabolic model generated for the same mammalian or bacterial cell expressing the same recombinant, heterologous polypeptide, and (ii) determining that the cultivation is affected by a problem if the modeled fit shows an offset with respect to the raw data of more than 10%, or the modeled fit has a chi2 value determined by a Pearson's chi-squared test of more than 5.