公开(公告)号：US20230081499A1

公开(公告)日：2023-03-16

申请号：US17992039

申请日：2022-11-22

Applicant: Hoffmann-La Roche Inc.

Inventor： Oliver POPP

IPC: C12N5/00 ,  C12N5/071

Abstract: The current invention reports the use of meta-tyrosine for increasing the specific productivity of a eukaryotic cell that produces/expresses a polypeptide. In the current method it is not necessary to perform a temperature-, osmolality- or pH shift or to add drugs like valproic acid or sodium butyrate to modulate the specific productivity of the cultivated cells. The method does not affect cell viability or product titer.

公开(公告)号：US20240327890A1

公开(公告)日：2024-10-03

申请号：US18741057

申请日：2024-06-12

Applicant: Hoffmann-La Roche Inc.

Inventor： Oliver POPP ,  Nicola BEAUCAMP ,  Georg DRABNER ,  Stephanie ESSLINGER

IPC: C12P21/00 ,  C07K16/00 ,  C12N1/38

CPC classification number: C12P21/005 ,  C07K16/00 ,  C12N1/38 ,  C07K2317/41

Abstract: The present invention relates to methods for selecting between conditions which enhance cell growth or biomass generation and conditions which affect the N-glycosylation maturity of expressed glycoprotein produced by eukaryotic cells under fermentation culture conditions. Thus, in the methods of the present invention, the glycoprotein producing cells are cultured in a medium which is tailored to a desired end result. The invention also embraces the medium and the use thereof.

公开(公告)号：US20210257045A1

公开(公告)日：2021-08-19

申请号：US17186816

申请日：2021-02-26

Applicant: Hoffmann-La Roche Inc.

Inventor： Tobias GROSSKOPF ,  Oliver POPP ,  Tobias WALLOCHA

IPC: G16B5/00 ,  G16B50/10

Abstract: Herein is reported a method for determining if process data acquired during the cultivation of a mammalian or bacterial cell is affected by a problem comprising the steps of (i) fitting the process data acquired during the cultivation of a mammalian or bacterial cell clone expressing a recombinant, heterologous polypeptide in a metabolic model generated for the same mammalian or bacterial cell expressing the same recombinant, heterologous polypeptide, and (ii) determining that the cultivation is affected by a problem if the modeled fit shows an offset with respect to the raw data of more than 10%, or the modeled fit has a chi2 value determined by a Pearson's chi-squared test of more than 5.

公开(公告)号：US20210222220A1

公开(公告)日：2021-07-22

申请号：US17006463

申请日：2020-08-28

Applicant: Hoffmann-La Roche Inc.

Inventor： Oliver POPP ,  Nicola BEAUCAMP ,  Georg DRABNER ,  Stephanie ESSLINGER

IPC: C12P21/00 ,  C12N1/38 ,  C07K16/00

Abstract: The present invention relates to methods for selecting between conditions which enhance cell growth or biomass generation and conditions which affect the N-glycosylation maturity of expressed glycoprotein produced by eukaryotic cells under fermentation culture conditions. Thus, in the methods of the present invention, the glycoprotein producing cells are cultured in a medium which is tailored to a desired end result. The invention also embraces the medium and the use thereof.