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公开(公告)号：US10702800B2
公开(公告)日：2020-07-07
申请号：US15440350
申请日：2017-02-23
Applicant: Hoffmann-La Roche Inc.
Inventor： Oskar Braunersreuther , Alexander Jockwer , Torsten Klabuhn , Michael Pohlscheidt , Joerg Thiele
Abstract: Separating apparatus, comprising a sedimentation settler and a collection vessel disposed underneath and being in fluid communication with the sedimentation settler, the collection vessel forming a receiving chamber having an outlet at or adjacent to the chamber bottom and having an inlet opening, wherein the collection vessel is arranged such the flow direction of the fluid in the area underneath the sedimentation settler is substantially in line with the direction of the channels of the sedimentation settler.
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Patent Agency Ranking
公开(公告)号：US20180010162A1
公开(公告)日：2018-01-11
申请号：US15713268
申请日：2017-09-22
Applicant: Hoffmann-La Roche Inc.
Inventor： Sylvia Baumann , Jens Hoffmann , Alexander Jockwer , Christian Klinger , Thomas Troebs
CPC classification number: C12P21/005 , B01F15/0479 , B01F2215/044 , C07K16/244 , C07K2317/14 , C12M29/26 , C12M41/26 , C12N5/00 , C12N2500/60
Abstract: Herein is reported a feed mixing device for adding feed solutions with a non-physiologically pH value to a cell cultivation vessel comprising a chamber for mixing the feed solutions prior to their addition to the cell cultivation vessel as well as its use. With the feed mixing device as reported herein feed components can be provided in solution at a pH value at which they have good solubility and/or good stability whereby the pH value can be clearly different from the pH value of the cultivation medium, i.e. different from the physiological pH value. This allows performing the cultivation with more flexibility compared to a cultivation in which the pH value of the feed solution is limited to a small range around the pH value of the cultivation.
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公开(公告)号：US20170157539A1
公开(公告)日：2017-06-08
申请号：US15440350
申请日：2017-02-23
Applicant: Hoffmann-La Roche Inc.
Inventor： Oskar Braunersreuther , Alexander Jockwer , Torsten Klabuhn , Michael Pohlscheidt , Joerg Thiele
CPC classification number: B01D21/0042 , B01D21/0045 , B01D21/0057 , B01D2221/06 , C12M27/02 , C12M27/20 , C12M33/22 , C12M47/02
Abstract: Separating apparatus, comprising a sedimentation settler and a collection vessel disposed underneath and being in fluid communication with the sedimentation settler, the collection vessel forming a receiving chamber having an outlet at or adjacent to the chamber bottom and having an inlet opening, wherein the collection vessel is arranged such the flow direction of the fluid in the area underneath the sedimentation settler is substantially in line with the direction of the channels of the sedimentation settler.
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公开(公告)号：US11697079B2
公开(公告)日：2023-07-11
申请号：US16899453
申请日：2020-06-11
Applicant: Hoffmann-La Roche Inc.
Inventor： Oskar Braunersreuther , Alexander Jockwer , Torsten Klabuhn , Michael Pohlscheidt , Joerg Thiele
CPC classification number: B01D21/0042 , B01D21/0045 , B01D21/0057 , C12M27/02 , C12M27/20 , C12M33/22 , C12M47/02 , B01D2221/06
Abstract: Separating apparatus, comprising a sedimentation settler and a collection vessel disposed underneath and being in fluid communication with the sedimentation settler, the collection vessel forming a receiving chamber having an outlet at or adjacent to the chamber bottom and having an inlet opening, wherein the collection vessel is arranged such the flow direction of the fluid in the area underneath the sedimentation settler is substantially in line with the direction of the channels of the sedimentation settler.
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公开(公告)号：US10513697B2
公开(公告)日：2019-12-24
申请号：US14540322
申请日：2014-11-13
Applicant: Hoffmann-La Roche Inc.
Inventor： Betina Da Silva Ribeiro , Markus Emmler , Alexander Jockwer
Abstract: A dissolved CO2 change in aqueous solutions affects directly the intracellular pH (pHi) value as it does so by influencing therefore important cellular processes. The enzyme carbonic anhydrase II (CAII) catalyzes the equilibrium of CO2 in aqueous solutions and because it alters the speed at which this equilibrium is reached it was identified as a strong candidate for metabolic engineering. The cell line stably expressing hCAII presented a better initial re-alkalinization of cytoplasm after induced CO2 acid load. The most alkaline pHi value associated to the lowest pHi variations was observed for that cell line in long term increased CO2 levels. In general, the increased CO2 profile triggered the quicker progress of G0G1-cell cycle phase for both transfected and control cell lines.
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公开(公告)号：US20140051121A1
公开(公告)日：2014-02-20
申请号：US13909591
申请日：2013-06-04
Applicant: Hoffmann-La Roche Inc.
Inventor： Sylvia Baumann , Jens Hoffmann , Alexander Jockwer , Christian Klinger , Thomas Troebs
CPC classification number: C12P21/005 , B01F15/0479 , B01F2215/044 , C07K16/244 , C07K2317/14 , C12M29/26 , C12M41/26 , C12N5/00 , C12N2500/60
Abstract: Herein is reported a feed mixing device for adding feed solutions with a non-physiologically pH value to a cell cultivation vessel comprising a chamber for mixing the feed solutions prior to their addition to the cell cultivation vessel as well as its use. With the feed mixing device as reported herein feed components can be provided in solution at a pH value at which they have good solubility and/or good stability whereby the pH value can be clearly different from the pH value of the cultivation medium, i.e. different from the physiological pH value. This allows performing the cultivation with more flexibility compared to a cultivation in which the pH value of the feed solution is limited to a small range around the pH value of the cultivation.
Abstract translation: 本文报道了一种用于将细胞培养容器中添加具有非生理学pH值的进料溶液加入到细胞培养容器中的饲料混合装置，所述培养容器包括用于将饲料溶液加入细胞培养容器之前混合饲料溶液的室以及其用途。 使用本文所述的进料混合装置，可以在溶液中以pH值提供给料组分，在该pH下它们具有良好的溶解性和/或良好的稳定性，因此pH值可以与培养基的pH值明显不同，即不同于 生理pH值。 与将培养液的pH值限制在培养pH值附近的小范围的培养相比，可以更有效地进行培养。
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