公开(公告)号：US20050097625A1

公开(公告)日：2005-05-05

申请号：US10722903

申请日：2003-11-26

申请人： Harry Meade ,  Eszter Birck-Wilson ,  Daniel Pollock

发明人： Harry Meade ,  Eszter Birck-Wilson ,  Daniel Pollock

IPC分类号： A01K67/027 ,  C07K16/04 ,  C12N15/85 ,  C12P21/00

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/01 ,  C07K16/04 ,  C07K2317/21 ,  C07K2317/41 ,  C07K2317/52 ,  C07K2317/53 ,  C12N15/8509 ,  C12N2830/008 ,  C12N2830/85

摘要： The invention features methods of producing an antibody in the milk of a transgenic mammal. The methods include providing a transgenic mammal whose somatic and germ cells comprise a sequence encoding an exogenous heavy chain variable region or antigen binding fragment thereof, at least one heavy chain constant region, or a fragment thereof, and a hinge region, operably linked to a promoter which directs expression in mammary epithelial cells, wherein said hinge region has been altered from the hinge region normally associated with the heavy chain constant region. The invention also features transgenic mammals, methods of producing these mammals, compositions comprising such antibodies, and nucleic acids encoding the antibodies.

摘要翻译： 本发明的特征在于在转基因哺乳动物的乳中产生抗体的方法。 所述方法包括提供转基因哺乳动物，其体细胞和生殖细胞包含编码外源重链可变区或其抗原结合片段的序列，至少一个重链恒定区或其片段和铰链区，其可操作地连接到 其指导乳腺上皮细胞中的表达，其中所述铰链区已经从通常与重链恒定区相关联的铰链区改变。 本发明还涉及转基因哺乳动物，产生这些哺乳动物的方法，包含此类抗体的组合物和编码抗体的核酸。