公开(公告)号：US12163171B2

公开(公告)日：2024-12-10

申请号：US16343675

申请日：2017-10-20

Applicant: HP Health Solutions Germany GmbH

Inventor： Federico Buersgens ,  Joachim Stehr ,  Lars Ullerich ,  Lidiya Osinkina ,  Eimantas Ruseckas

IPC: C12P19/34 ,  B01J19/08 ,  B01L7/00 ,  C12Q1/686

Abstract: A method for amplifying nucleic acids by a polymerase chain reaction in a reaction volume heated using electrical energy. In at least one of the passages of the amplification cycle of the polymerase chain reaction, the ratio of the electrical energy used in the denaturation step to heat the reaction volume to the size of the reaction volume is less than 20 Joule per milliliter. Further shown is a method of amplifying nucleic acids in a reaction volume by using a device that includes a reaction vessel and a heating means with at least one heating element in contact with the reaction volume where at least one heating element is conjugated to oligonucleotides. Also shown is a device for the amplification of nucleic acids in a reaction volume including a reaction vessel for receiving the reaction volume and a heating means consisting of at least one heating element contacting the reaction volume.