公开(公告)号：US20140178996A1

公开(公告)日：2014-06-26

申请号：US14108409

申请日：2013-12-17

申请人： HITACHI, LTD.

发明人： Keisuke SHIBUYA ,  Ryoichi HAGA ,  Masaru NAMBA ,  Kenichiro OKA ,  Ken AMANO

IPC分类号： C12M1/34

CPC分类号： C12M41/42 ,  C12M25/02 ,  C12M27/02 ,  C12M35/04 ,  C12M41/32 ,  C12M41/48

摘要： This invention provides a culture control method and a cell culture apparatus that enable stable cell culture by regulating shear stress to be applied to cells within an adequate range. With the application of such culture control method and cell culture apparatus, cell culture is performed under agitation culture conditions in which the shear stress distribution is 0.5 Pa to 20 Pa in 80% or more of the culture vessel by volume.

摘要翻译： 本发明提供一种培养控制方法和细胞培养装置，其通过调节施加到适当范围内的细胞的剪切应力来实现稳定的细胞培养。 通过这样的培养控制方法和细胞培养装置的应用，在培养容器的体积的80％以上的剪切应力分布为0.5Pa〜20Pa的搅拌条件下进行细胞培养。