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公开(公告)号：US20230374497A1
公开(公告)日：2023-11-23
申请号：US18176062
申请日：2023-02-28
发明人： Amy SHEN , Inn Huam Yuk , Peggy Wai No Ko , Shahram Misaghi , Simon Thomas Auslaender , Midori Greenwood-Goodwin , Michael Wilson Laird , Benedikt Alois Claudius Oswald
CPC分类号： C12N15/1082 , C12N15/11 , C12N9/22 , C12N15/907 , C12N5/00 , C12N2310/20 , C12N2800/80
摘要： The present disclosure relates to modified mammalian cells having reduced or eliminated expression of certain cellular proteins, CRISPR/Cas9 multiplex knockout strategies for making such cells, and methods of using such cells, e.g., in the context of cell-based therapy or as host cells in the production of a product of interest.
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公开(公告)号：US20240254199A1
公开(公告)日：2024-08-01
申请号：US18489274
申请日：2023-10-18
发明人： Amy Shen , Inn Huam Yuk , Gavin Christian Barnard , Shahram Misaghi , Simon Auslaender , Niels Bauer , Benedikt Oswald
CPC分类号： C07K16/00 , C12N5/00 , C12N9/22 , C12N15/11 , C12N15/907 , C12P21/00 , C12N2310/20 , C12N2800/80
摘要： The present disclosure relates to mammalian cells (e.g., Chinese Hamster Ovary (CHO) cells) that are modified to reduce or eliminate the expression of certain mammalian cell endogenous products (e.g., host cell proteins and virus-like particles), and methods of using such cells in the production of a recombinant product of interest, e.g., a recombinant protein, a recombinant viral particle, or a recombinant viral vector. These modifications were specifically chosen to generate engineered mammalian host cells with desired traits in several key areas, including improved cell culture performance (e.g., higher viability and product titers), improved product quality (e.g., more consistent and favorable glycosylation; more stable drug product), and decreased burden on purification for removing problematic or undesired endogenous host cell products (e.g., hydrolytic host cell proteins and virus-like particles) during biomanufacturing.
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公开(公告)号：US20240168036A1
公开(公告)日：2024-05-23
申请号：US18526020
申请日：2023-12-01
发明人： Jia Guo , Regina Kufer , Delia Li , Feng Yang , Inn Huam Yuk , Jinhua Zhang , Lance J. Cadang , Lu Dai
CPC分类号： G01N33/6848 , G01N1/4044 , G01N30/7233 , G01N30/88 , G01N2030/8831
摘要： The present disclosure relates to highly sensitive methods for determining the identity and quantity of one or more proteins in a sample. For example, the present disclosure provides methods for the highly sensitive identification and quantitation of residual host cell proteins in protein samples and can be adapted to identify and quantify proteins in either a targeted or a target-agnostic manner and can be modified to achieve a range of sensitivities appropriate for distinct use cases.
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公开(公告)号：US20240011066A1
公开(公告)日：2024-01-11
申请号：US18303451
申请日：2023-04-19
发明人： Shahram Misaghi , Amy Shen , Inn Huam Yuk , Gavin Christian Barnard , Simon Auslaender , Niels Bauer , Benedikt Oswald
CPC分类号： C12P21/02 , C12N15/907 , C12N15/11 , C12N9/93 , C12N9/22 , C12N9/1029 , C12Y203/01 , C12N9/86 , C12Y305/02006 , C12N2310/20 , C12N2800/80
摘要： Herein are reported modified mammalian cells wherein the expression of the MYC gene and one or more of the BAK, BAX, SIRT-1 and ICAM-1 genes has been reduced or eliminated as well as methods for the recombinant production of a heterologous polypeptide using a modified mammalian cell according to the current invention. Further reported are use of the reduction of the expression of the genes for increasing volumetric productivity, increasing cell volume, increasing viability and increasing the possible cultivation time without cell split.
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公开(公告)号：US12098365B2
公开(公告)日：2024-09-24
申请号：US17213611
申请日：2021-03-26
申请人： GENENTECH, INC.
CPC分类号： C12N15/102 , C12N5/0682 , C12N2510/00
摘要： The present disclosure relates to methods, cells, and compositions for producing a product of interest, e.g., a recombinant protein. In particular, the present disclosure provides improved mammalian cells expressing the product of interest, where the cells (e.g., Chinese Hamster Ovary (CHO) cells) have reduced or eliminated activity, e.g., expression, of certain host cell proteins, e.g., enzymes including, but not limited to, certain lipases, esterases, and/or hydrolases.
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公开(公告)号：US20210230658A1
公开(公告)日：2021-07-29
申请号：US17221378
申请日：2021-04-02
申请人： GENENTECH, INC.
IPC分类号： C12P21/00
摘要： A method of reducing glycation of an amino acid in a protein produced by a host cell in cell culture medium is disclosed.
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