公开(公告)号：US5998330A

公开(公告)日：1999-12-07

申请号：US803670

申请日：1997-02-21

Applicant: Gary W. Felton

Inventor： Gary W. Felton

IPC: A01N63/00 ,  A01N35/06 ,  C07C50/00

CPC classification number: A01N63/00

Abstract: A method of treating vegetation by application of a microbial insecticide in which a quinone has been covalently bonded to the viral occlusion body surface of the microbial insecticide in order to improve the UV stability of the microbial insecticide by forming a protective shield around the pathogen.

Abstract translation: 通过应用微生物杀虫剂处理植物的方法，其中醌已经共价结合到微生物杀虫剂的病毒闭塞体表面，以通过在病原体周围形成保护屏蔽来改善微生物杀虫剂的UV稳定性。

公开(公告)号：US06303326B1

公开(公告)日：2001-10-16

申请号：US09205630

申请日：1998-12-03

Applicant: Gary W. Felton ,  Mary C. Mathews ,  Jianlong Bi ,  John B. Murphy

Inventor： Gary W. Felton ,  Mary C. Mathews ,  Jianlong Bi ,  John B. Murphy

IPC: C12Q154

CPC classification number: C12N9/0006 ,  A01N37/46 ,  A01N63/02 ,  C12Q1/26 ,  Y10S435/805 ,  Y10S435/808 ,  Y10S435/814 ,  Y10S435/815 ,  Y10T436/144444

Abstract: The present invention includes the characterization of the major salivary protein or enzyme of the corn earworm Helicoverpa zea for triggering resistance to bacterial blight and frogeye leafspot in soybeans and for triggering resistance to insects in tomatoes. The invention includes an enzyme or a novel protein secreted from the salivary glands of certain insects including the saliva of species belonging to the order Hymenoptera and Lepidoptera. The regurgitant of Helocoverpa zea obtained from the functional salivary glands contains a protein that possesses glucose oxidase activity. The amino acid sequence of the protein is unique and when the protein is applied to plants, it triggers disease and insect resistance systematically. The physical and kinetic attributes of the enzyme are a pH of 7.0, a pI of 4.4 and a molecular weight of 88 kd. The km and Vmax of the enzyme for glucose is 26.9 mmol and 26.7 &mgr;mol min−1 mg−1, respectively. The enzyme may be expressed in crops for plant resistance and/or applied to crops for inhibiting foliar pathogens and/or other pests, in food applications for improving the shelf life or quality of the food products as well as the production of low alcohol products. Biomedical uses include using the enzyme for glucose monitoring of blood, urine, etc., as well as using the enzyme in the development of anti-cancer and/or anti-tumor agents and the production of antimicrobial products and the like. Various biochemical applications include the use of the enzyme in immunochemistry as well as for the enzymatic iodination of proteins and enzymatically amplified sensors for amperometry and voltammetry. Another use of the gene from the enzyme is an expression in a Baculovirus for pesticide usage.

Abstract translation: 本发明包括玉米螟Helicoverpa zea的主要唾液蛋白或酶的特征，用于触发对大豆中的细菌性枯萎病和青蛙叶斑病的抗性，并触发对番茄中的昆虫的抗性。 本发明包括从某些昆虫的唾液腺分泌的酶或新型蛋白质，包括属于膜翅目和鳞翅目的物种的唾液。 从功能性唾液腺获得的Helocoverpa zea的反流剂含有具有葡萄糖氧化酶活性的蛋白质。 蛋白质的氨基酸序列是独特的，当蛋白质应用于植物时，系统地触发疾病和昆虫抗性。 酶的物理和动力学特性是pH为7.0，pI为4.4，分子量为88kd。 葡萄糖酶的km和Vmax分别为26.9 mmol和26.7 molol min-1 mg-1。 该酶可以在用于植物抗性的作物中表达和/或施用于作物以抑制叶病原体和/或其它害虫，在食品应用中用于改善食品的保质期或质量以及生产低级酒精产品。 生物医学用途包括使用酶进行血液，尿液等的葡萄糖监测，以及在开发抗癌和/或抗肿瘤剂中使用该酶以及生产抗微生物产品等。 各种生物化学应用包括在免疫化学中使用酶以及蛋白质的酶碘化和用于电流分析和伏安法的酶扩增传感器。 来自酶的基因的另一个用途是在用于农药使用的杆状病毒中的表达。