公开(公告)号：US09156030B2

公开(公告)日：2015-10-13

申请号：US13713954

申请日：2012-12-13

Applicant: Eppendorf AG

Inventor： Jochen Beese

IPC: B01L3/02

CPC classification number: B01L3/021 ,  B01L3/0275 ,  B01L3/0279 ,  B01L2200/0689

Abstract: A pipette tip having the shape of an elongate small tube with a lower opening at the lower end for the passage of liquid, and an upper opening at the upper end, wherein a seat area exists on the inner circumference contiguous to the upper opening, which serves for plugging it onto a standardized conical attachment piece of a pipetting device, wherein the seat area has a retaining area with axially extending ribs projecting radially towards the inside, and below the retaining area a sealing area and is configured such that when the pipette tip is plugged onto the attachment piece with its seat area by a plugging force which ensures retaining and sealing of the pipette tip on the attachment piece, the ribs are partly plastically deformed, and elastic deformation occurs in the seat area outside of the ribs.

Abstract translation: 具有细长小管的形状的移液管尖端，在下端具有用于液体通过的下开口，以及在上端的上开口，其中在与上开口相邻的内周上存在座区， 用于将其堵塞到移液装置的标准化锥形附接件上，其中座位区域具有保持区域，其具有径向朝向内部突出的轴向延伸的肋部，并且在保持区域的下方形成密封区域，并且构造成使得当移液管吸头 通过堵塞力将塞子面积堵塞在附件上，该堵塞力确保移液管尖端在附件上的保持和密封，肋部分地塑性变形，并且在肋的外侧的座位区域发生弹性变形。

公开(公告)号：US10030220B2

公开(公告)日：2018-07-24

申请号：US14408069

申请日：2013-06-13

Applicant: EPPENDORF AG

Inventor： Matthias Arnold ,  Heinz Gerhard Kohn ,  Nico Gulzow ,  Sven Eikelmann ,  Sebastian Selzer ,  Jochen Beese ,  Christopher Gunther ,  Eckart Kopowski

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/06

Abstract: A single-use bioreactor, suitable in particular for use in a parallel bioreactor system for applications in cell culture and/or microbiology, a head plate for such a single-use bioreactor and a process for manufacturing the single-use bioreactor and the head plate. The single-use bioreactor comprises a head plate, a dimensionally stable container and a mixer, the head plate and the container enclosing a reaction chamber, and the mixer having a mixer shaft and a stirring member, said mixer shaft being mounted rotatably about a rotational axis in a bearing and the stirring member being fastened torsionally rigidly to the mixer shaft. The mixer and the bearing are arranged entirely within the reaction chamber and the mixer shaft has a magnetic portion which is arranged and adapted in such a way that it can be coupled magnetically in an axial direction to a rotary drive.

公开(公告)号：US20190031995A1

公开(公告)日：2019-01-31

申请号：US16072630

申请日：2017-01-27

Applicant: EPPENDORF AG

Inventor： Matthias Arnold ,  Sebastian Selzer ,  Sven Eikelmann ,  Jochen Beese

IPC: C12M1/00 ,  C12M1/12

Abstract: The invention relates to a single-use connection device for insertion into a connection opening of a bioreactor, in particular in a connection opening of a top plate of a dimensionally-stable bioreactor or in a connection opening of a connection element of a pouch bioreactor. The single-use connection device is inserted in a connection opening of a top plate of a bioreactor and/or in a connection opening of a pouch reactor and is provided with multiple passages and an attachment section, wherein the single-use connection device is formed as a single piece and is made of plastic. The attachment section, on an outer peripheral surface, has an attachment structure for attaching the single-use connection device in the connection opening.

公开(公告)号：US20130213151A1

公开(公告)日：2013-08-22

申请号：US13713954

申请日：2012-12-13

Applicant: Eppendorf AG

Inventor： Jochen Beese

IPC: B01L3/02

CPC classification number: B01L3/021 ,  B01L3/0275 ,  B01L3/0279 ,  B01L2200/0689

Abstract: A pipette tip having the shape of an elongate small tube with a lower opening at the lower end for the passage of liquid, and an upper opening at the upper end, wherein a seat area exists on the inner circumference contiguous to the upper opening, which serves for plugging it onto a standardized conical attachment piece of a pipetting device, wherein the seat area has a retaining area with axially extending ribs projecting radially towards the inside, and below the retaining area a sealing area and is configured such that when the pipette tip is plugged onto the attachment piece with its seat area by a plugging force which ensures retaining and sealing of the pipette tip on the attachment piece, the ribs are partly plastically deformed, and elastic deformation occurs in the seat area outside of the ribs.

Abstract translation: 具有细长小管的形状的移液管尖端，在下端具有用于液体通过的下开口，以及在上端的上开口，其中在与上开口相邻的内周上存在座区， 用于将其堵塞到移液装置的标准化锥形附接件上，其中座位区域具有保持区域，其具有径向朝向内部突出的轴向延伸的肋部，并且在保持区域的下方形成密封区域，并且构造成使得当移液管吸头 通过堵塞力将塞子面积堵塞在附件上，该堵塞力确保移液管尖端在附件上的保持和密封，肋部分地塑性变形，并且在肋的外侧的座位区域发生弹性变形。

公开(公告)号：US11608484B2

公开(公告)日：2023-03-21

申请号：US16072630

申请日：2017-01-27

Applicant: EPPENDORF AG

Inventor： Matthias Arnold ,  Sebastian Selzer ,  Sven Eikelmann ,  Jochen Beese

IPC: C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  C12M3/00

Abstract: The invention relates to a single-use connection device for insertion into a connection opening of a bioreactor, in particular in a connection opening of a top plate of a dimensionally-stable bioreactor or in a connection opening of a connection element of a pouch bioreactor. The single-use connection device is inserted in a connection opening of a top plate of a bioreactor and/or in a connection opening of a pouch reactor and is provided with multiple passages and an attachment section, wherein the single-use connection device is formed as a single piece and is made of plastic. The attachment section, on an outer peripheral surface, has an attachment structure for attaching the single-use connection device in the connection opening.

公开(公告)号：US20150132840A1

公开(公告)日：2015-05-14

申请号：US14408069

申请日：2013-06-13

Applicant: EPPENDORF AG

Inventor： Matthias Arnold ,  Heinz-Gerhard Kohn ,  Nico Gulzow ,  Eikelmann Sven ,  Sebastian Selzer ,  Jochen Beese ,  Christoph Gunther ,  Eckhart Kopowski

IPC: C12M1/00 ,  C12M1/06 ,  C12M3/00

CPC classification number: C12M23/28 ,  C12M23/40 ,  C12M23/42 ,  C12M27/02 ,  Y10T29/49826 ,  Y10T156/10

Abstract: A single-use bioreactor, suitable in particular for use in a parallel bioreactor system for applications in cell culture and/or microbiology, a head plate for such a single-use bioreactor and a process for manufacturing the single-use bioreactor and the head plate. The single-use bioreactor comprises a head plate, a dimensionally stable container and a mixer, the head plate and the container enclosing a reaction chamber, and the mixer having a mixer shaft and a stirring member, said mixer shaft being mounted rotatably about a rotational axis in a bearing and the stirring member being fastened torsionally rigidly to the mixer shaft. The mixer and the bearing are arranged entirely within the reaction chamber and the mixer shaft has a magnetic portion which is arranged and adapted in such a way that it can be coupled magnetically in an axial direction to a rotary drive.

Abstract translation: 适用于特别用于细胞培养和/或微生物学应用的平行生物反应器系统的一次性生物反应器，用于这种一次性生物反应器的头板和用于制造一次性生物反应器和头板的方法 。 一次性生物反应器包括头板，尺寸稳定的容器和混合器，头板和容器包围反应室，混合器具有混合器轴和搅拌构件，所述混合器轴围绕转动 轴，并且搅拌构件被扭转地刚性地紧固到混合器轴。 混合器和轴承完全布置在反应室内，混合器轴具有磁性部分，该磁性部分被布置和适配，使得其可以沿轴向方向磁耦合到旋转驱动器。