公开(公告)号：US20140178884A1

公开(公告)日：2014-06-26

申请号：US14118502

申请日：2012-05-22

申请人： David Aucoin ,  Thomas R. Kozel ,  Sindy Chaves

发明人： David Aucoin ,  Thomas R. Kozel ,  Sindy Chaves

IPC分类号： G01N33/569

CPC分类号： G01N33/56961 ,  G01N33/6893 ,  G01N2333/38 ,  G01N2800/52 ,  G01N2800/54

摘要： Disclosed herein are methods of diagnosing, monitoring and treating an Aspergillus species-associated condition, such as Aspergillus fumigatus (Af)-associated condition, including aspergillosis, such as invasive aspergillosis (IA). Methods for diagnosing and/or monitoring an Aspergillus species-associated condition, such as IA are provided. Also disclosed are point-of-care immunoassays that can be used to diagnose or monitor the efficacy of an Aspergillus-associated condition treatment. These immunoassays can also be used for rapid diagnosis of infection produced by an Aspergillus species, such as Af.

摘要翻译： 本文公开了诊断，监测和治疗曲霉属物种相关病症的方法，例如烟曲霉（Af）相关病症，包括曲霉病，例如侵袭性曲霉病（IA）。 提供了用于诊断和/或监测曲霉属物种相关病症如IA的方法。 还公开了可用于诊断或监测曲霉相关病症治疗的功效的点护理免疫测定。 这些免疫测定也可以用于快速诊断由曲霉属物种如Af产生的感染。

公开(公告)号：US09310365B2

公开(公告)日：2016-04-12

申请号：US13639450

申请日：2011-04-12

申请人： Thomas Kozel ,  David Aucoin

发明人： Thomas Kozel ,  David Aucoin

IPC分类号： G01N33/569 ,  C07K16/12

CPC分类号： G01N33/56911 ,  C07K16/1214 ,  G01N2333/21 ,  G01N2800/52

摘要： Non-invasive methods are provided herein for the diagnosis of melioidosis with specific antibodies capable of detecting molecules associated with melioidosis in a biological fluid, such as urine or serum. These molecules can be identified using proteomic methods, including but not limited to antibody based methods, such as an enzyme-linked immunosorbant assay (ELISA), a radioimmunoassay (RIA), or a lateral flow immunoassay. Methods of inducing an immune response to melioidosis are also disclosed. The methods include the use of the immunogenic melioidosis polypeptides, nucleic acids encoding these polypeptides, and/or viral vectors encoding an immunogenic melioidosis polypeptide, alone or in conjunction with other agents, such as traditional melioidosis therapies. Also disclosed are methods for treating a subject having melioidosis. These methods include inducing an immune response to melioidosis and/or using an inhibitory nucleic acid, such as a siRNA or antisense molecule, to decrease a melioidosis associated molecule expression in order to treat melioidosis.

摘要翻译： 本文提供了非侵入性方法，用于诊断具有能够检测与生物流体例如尿液或血清中的黑素综合征有关的分子的特异性抗体的嗜酸性粒细胞减少症。 可以使用蛋白质组学方法鉴定这些分子，包括但不限于基于抗体的方法，例如酶联免疫吸附测定（ELISA），放射免疫测定（RIA）或侧流免疫测定。 还公开了诱导对嗜酸性粒细胞减少症的免疫应答的方法。 所述方法包括单独使用免疫原性黑麦病多糖，编码这些多肽的核酸和/或编码免疫原性黑麦病多肽的病毒载体，或与其它试剂例如传统的黑素综合征疗法联合使用。 还公开了用于治疗患有黑痴病的受试者的方法。 这些方法包括诱导针对嗜酸性粒细胞减少症的免疫应答和/或使用抑制性核酸如siRNA或反义分子来降低与相似的分子表达的melioidosis相关的分子表达以治疗黑麦病。

公开(公告)号：US20130028927A1

公开(公告)日：2013-01-31

申请号：US13639450

申请日：2011-04-12

申请人： Thomas Kozel ,  David Aucoin

发明人： Thomas Kozel ,  David Aucoin

IPC分类号： A61K39/07 ,  A61P31/04 ,  A61K31/7088 ,  G01N21/64 ,  G01N33/566

CPC分类号： G01N33/56911 ,  C07K16/1214 ,  G01N2333/21 ,  G01N2800/52

摘要： Non-invasive methods are provided herein for the diagnosis of melioidosis with specific antibodies capable of detecting molecules associated with melioidosis in a biological fluid, such as urine or serum. These molecules can be identified using proteomic methods, including but not limited to antibody based methods, such as an enzyme-linked immunosorbant assay (ELISA), a radioimmunoassay (RIA), or a lateral flow immunoassay. Methods of inducing an immune response to melioidosis are also disclosed. The methods include the use of the immunogenic melioidosis polypeptides, nucleic acids encoding these polypeptides, and/or viral vectors encoding an immunogenic melioidosis polypeptide, alone or in conjunction with other agents, such as traditional melioidosis therapies. Also disclosed are methods for treating a subject having melioidosis. These methods include inducing an immune response to melioidosis and/or using an inhibitory nucleic acid, such as a siRNA or antisense molecule, to decrease a melioidosis associated molecule expression in order to treat melioidosis.

摘要翻译： 本文提供了非侵入性方法，用于诊断具有能够检测与生物流体例如尿液或血清中的黑素综合征有关的分子的特异性抗体的嗜酸性粒细胞减少症。 可以使用蛋白质组学方法鉴定这些分子，包括但不限于基于抗体的方法，例如酶联免疫吸附测定（ELISA），放射免疫测定（RIA）或侧流免疫测定。 还公开了诱导对嗜酸性粒细胞减少症的免疫应答的方法。 所述方法包括单独使用免疫原性黑麦病多糖，编码这些多肽的核酸和/或编码免疫原性黑麦病多肽的病毒载体，或与其它试剂例如传统的黑素综合征疗法联合使用。 还公开了用于治疗患有黑痴病的受试者的方法。 这些方法包括诱导针对嗜酸性粒细胞减少症的免疫应答和/或使用抑制性核酸如siRNA或反义分子来降低与相似的分子表达的melioidosis相关的分子表达以治疗黑麦病。