公开(公告)号：US20180051302A1

公开(公告)日：2018-02-22

申请号：US15550472

申请日：2015-06-25

Applicant: DALIAN NATIONALITIES UNIVERSITY

Inventor： Shubiao ZHANG ,  Yinan ZHAO ,  Shaohui CUI ,  Huiying CHEN ,  Quan ZHOU

IPC: C12N15/88 ,  A61K9/127 ,  C07K4/00 ,  C07K1/06

CPC classification number: C12N15/88 ,  A61K9/1272 ,  A61K47/542 ,  C07K1/06 ,  C07K1/066 ,  C07K4/00 ,  C07K5/06086 ,  C07K5/0815 ,  C07K5/1019

Abstract: A bis-alkoxyl amide alkyl cationic peptide lipid has a chemical structure as below: wherein the bis-alkoxyl amide alkyl cationic peptide lipid is dispersed in water to obtain the cationic peptide liposome which are high in stability and uniform in dispersion and have about 120 nm of average grain diameter and Zeta electric potential between 30 and 50 mV. The liposome can effectively compress the plasmids DNA and siRNA, can efficient transfection both in-vitro and in-vivo, and almost does not have toxicity to cells and mice, so that the liposome can be widely applied in gene delivery as a gene vector.

公开(公告)号：US20170355667A1

公开(公告)日：2017-12-14

申请号：US15542268

申请日：2015-06-25

Applicant: DALIAN NATIONALITIES UNIVERSITY

Inventor： Shubiao ZHANG ,  Yinan ZHAO ,  Shaohui CUI ,  Defu ZHI ,  Hua HAI

IPC: C07C269/06 ,  C09K17/02 ,  C07C271/20 ,  C12N15/87 ,  C07K5/00

CPC classification number: C07C269/06 ,  C07C271/20 ,  C07K5/00 ,  C09K17/02 ,  C12N15/87 ,  C12N15/88

Abstract: A class of propyl cationic peptide lipids is propyl cationic peptide lipid compounds having a general formula structure as follows. After the propyl cationic peptide lipids are dispersed in water, a cationic liposome with a particle size of approximately 100 nm is obtained. The cationic liposome can carry plasmid DNA (pDNA) or small interfering RNA (siRNA) into cells to realize the function of gene delivery, and is almost non-toxic to the cells.

公开(公告)号：US20170326255A1

公开(公告)日：2017-11-16

申请号：US15546942

申请日：2015-06-25

Applicant: DALIAN NATIONALITIES UNIVERSITY

Inventor： Shubiao ZHANG ,  Defu ZHI ,  Shaohui CUI ,  Yinan ZHAO ,  Quan ZHOU

IPC: A61K48/00 ,  A61K9/127 ,  C12N15/88 ,  A61K47/26 ,  A61K9/107 ,  C07H5/02 ,  A61K47/28 ,  C07J17/00 ,  C12N15/113

CPC classification number: A61K48/0033 ,  A61K9/1075 ,  A61K9/127 ,  A61K9/1272 ,  A61K47/26 ,  A61K47/28 ,  C07H1/00 ,  C07H5/02 ,  C07H13/06 ,  C07J17/005 ,  C12N15/111 ,  C12N15/113 ,  C12N15/88 ,  C12N2310/14 ,  C12N2320/32

Abstract: The invention provides a class of sucrose ester based cationic lipids and preparation method thereof. The vector prepared using the cationic lipid can be used to deliver nucleic acid. The sucrose ester based cationic lipid is prepared by using a chemical synthesis method in the invention, wherein the synthesis method is simple, and has a relatively high product yield. A composition, including suspension, emulsion, micelle and liposome and the like, can be prepared by mixing the sucrose ester based cationic lipid compound of the invention with a co-lipid. Sucrose ester based cationic lipid complex can be prepared by using the said composition and nucleic acid, which has advantages such as simple preparation, low toxicity and high transfection efficiency, and is a novel and highly efficient gene vector.