公开(公告)号：US20040046956A1

公开(公告)日：2004-03-11

申请号：US10658363

申请日：2003-09-08

Applicant: Chromagen, Inc.

Inventor： Gene Gould ,  Michael J. Conrad

IPC: G01J003/12

CPC classification number: G01J3/06 ,  G01J1/0437 ,  G01J3/02 ,  G01J3/12 ,  G01J3/18 ,  G01J3/1838 ,  G01J3/4406 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6417 ,  G01N2021/6463 ,  G01N2021/6482

Abstract: A fluorescence spectrophotometer having an excitation double monochromator, a coaxial excitation/emission light transfer module, and an emission double monochromator. Each monochromator includes a pair of holographic concave gratings mounted to precisely select a desired band of wavelengths from incoming broadband light without using other optical elements, such as mirrors. Selected excitation light is directed into a sample well by a light transfer module that includes a coaxial excitation mirror positioned to direct excitation light directly to the bottom of a well of a multi-well plate. Fluorescence emission light that exits the well opening is collected by a relatively large coaxial emission mirror. The collected emission light is wavelength selected by the emission double monochromator. Selected emission light is detected by a photodetector module.

Abstract translation: 具有激发双色单色器，同轴激发/发射光传输模块和发射双色单色仪的荧光分光光度计。 每个单色器包括一对全息凹面光栅，其安装成从入射的宽带光线精确地选择所需的波长带，而不使用诸如反射镜的其它光学元件。 所选择的激发光通过光传输模块被引导到样品阱中，所述光传输模块包括同轴激发反射镜，其被定位成将激发光直接引导到多孔板的阱的底部。 离开井口的荧光发射光由较大的同轴发射镜收集。 所收集的发射光是由发射双色仪选择的波长。 所选择的发射光由光电检测器模块检测。