公开(公告)号：US09494781B2

公开(公告)日：2016-11-15

申请号：US13352752

申请日：2012-01-18

Applicant: Chin-Lin Guo ,  Jiun-Yann Yu ,  Chun-Hung Kuo

Inventor： Chin-Lin Guo ,  Jiun-Yann Yu ,  Chun-Hung Kuo

IPC: G02B21/06

CPC classification number: G02B21/06 ,  G02B2207/114

Abstract: A novel method and system for conducting wide-field multi-photon microscopy through plane-projection are provided. It has been discovered that the limitations of conventional temporal-focusing techniques, such as single excitation wavelength and low acquisition rates can be resolved utilizing a novel optical set-up in which an optical diffuser is used as the scatterer. The use of such an optical arrangement enables temporal focusing regardless of the central wavelength of laser pulses. In addition, the optical sectioning possible using the disclosed microscopy is comparable to confocal microscopy, and can be robustly achieved by both moderate and high NA objectives at 100-fs pulse width. Moreover, the multi-photon excitation efficiency of the disclosed system can be enhanced by lowering the repetition rate of the ultrafast laser light source at constant pulse width and average power.

Abstract translation: 提供了一种通过平面投影进行宽场多光子显微镜的新方法和系统。 已经发现，使用光扩散器作为散射体的新型光学装置，可以解决诸如单激发波长和低采集速率的常规时间聚焦技术的局限性。 使用这种光学布置能够实现时间聚焦，而不管激光脉冲的中心波长如何。 此外，使用公开的显微镜可能的光学切片可与共焦显微镜相当，并且可以通过在100-fs脉冲宽度下的中NA NA物镜和高NA物镜来稳健地实现。 此外，通过以恒定的脉冲宽度和平均功率降低超快激光光源的重复率，可以提高所公开的系统的多光子激发效率。

公开(公告)号：US20120182413A1

公开(公告)日：2012-07-19

申请号：US13352752

申请日：2012-01-18

Applicant: Chin-Lin Guo ,  Jiun-Yann Yu ,  Chun-Hung Kuo

Inventor： Chin-Lin Guo ,  Jiun-Yann Yu ,  Chun-Hung Kuo

IPC: H04N7/18

CPC classification number: G02B21/06 ,  G02B2207/114

Abstract: A novel method and system for conducting wide-field multi-photon microscopy through plane-projection are provided. It has been discovered that the limitations of conventional temporal-focusing techniques, such as single excitation wavelength and low acquisition rates can be resolved utilizing a novel optical set-up in which an optical diffuser is used as the scatterer. The use of such an optical arrangement enables temporal focusing regardless of the central wavelength of laser pukes. In addition, the optical sectioning possible using the disclosed microscopy is comparable to confocal microscopy, and can be robustly achieved by both moderate and high NA objectives at 100-fs puke width. Moreover, the multi-photon excitation efficiency of the disclosed system can be enhanced by lowering the repetition rate of the ultrafast laser light source at constant pulse width and average power.

Abstract translation: 提供了一种通过平面投影进行宽场多光子显微镜的新方法和系统。 已经发现，使用光扩散器作为散射体的新型光学装置，可以解决诸如单激发波长和低采集速率的常规时间聚焦技术的局限性。 使用这种光学布置能够实现时间聚焦，而与激光脉冲的中心波长无关。 此外，使用公开的显微镜可能的光学切片可与共聚焦显微镜相当，并且可以通过100-fs浦口宽度的中度和高NA目标得到鲁棒地实现。 此外，通过以恒定的脉冲宽度和平均功率降低超快激光光源的重复率，可以提高所公开的系统的多光子激发效率。

公开(公告)号：US07773278B2

公开(公告)日：2010-08-10

申请号：US12483026

申请日：2009-06-11

Applicant: Shi-Wei Chu ,  Jiun-Yann Yu

Inventor： Shi-Wei Chu ,  Jiun-Yann Yu

IPC: G02B26/08

CPC classification number: G02B21/002 ,  G02B26/101 ,  G03F7/70233 ,  G03F7/70383

Abstract: The present invention is a scanning system having spherical mirrors to operate a two-dimensional scanning. Aberration owing to oblique incident light is compensated to reach diffraction limit. With proper metallic coating, the present invention uses a wavelength from ultra-violet light to tera-hertz wavelength

Abstract translation: 本发明是具有用于操作二维扫描的球面镜的扫描系统。 由斜入射光引起的畸变得到补偿，达到衍射极限。 通过适当的金属涂层，本发明使用从紫外光到紫外线波长的波长

公开(公告)号：US20100118365A1

公开(公告)日：2010-05-13

申请号：US12483026

申请日：2009-06-11

Applicant: Shi-Wei Chu ,  Jiun-Yann Yu

Inventor： Shi-Wei Chu ,  Jiun-Yann Yu

IPC: G02B26/12 ,  G02B26/10

CPC classification number: G02B21/002 ,  G02B26/101 ,  G03F7/70233 ,  G03F7/70383

Abstract: The present invention is a scanning system having spherical mirrors to operate a two-dimensional scanning. Aberration owing to oblique incident light is compensated to reach diffraction limit. With proper metallic coating, the present invention uses a wavelength from ultra-violet light to tera-hertz wavelength

Abstract translation: 本发明是具有用于操作二维扫描的球面镜的扫描系统。 由斜入射光引起的畸变得到补偿，达到衍射极限。 通过适当的金属涂层，本发明使用从紫外光到紫外线波长的波长