公开(公告)号：US09932551B2

公开(公告)日：2018-04-03

申请号：US15463448

申请日：2017-03-20

Applicant: CPSI Holdings, LLC

Inventor： John G. Baust ,  Joshua T. Smith ,  Kimberly L. Santucci ,  Kristi K. Snyder ,  Anthony T. Robilotto ,  Robert G. Van Buskirk ,  John M. Baust ,  William L. Corwin ,  Jennie F. McKain

IPC: C12M1/14 ,  C12M3/00 ,  C12M1/12 ,  C12M1/02

CPC classification number: C12M21/08 ,  C12M25/06 ,  C12M25/14 ,  C12M27/00 ,  C12M29/00 ,  C12N5/0062 ,  C12N5/0068 ,  C12N5/0697 ,  C12N2537/00 ,  G01N33/5008 ,  G01N33/5017

Abstract: A tissue engineered model (TEM) structure, an apparatus and method for making a TEM structure, and methods of using a TEM structure are disclosed. In an embodiment, the TEM structure includes at least one TEM segment. Each TEM segment includes a frame defining a bounded area, the frame having a height, a first edge, and a second edge opposite the first edge, each of the first edge and the second edge defining a perimeter of the bounded area, and the height defining a distance between the first edge and the second edge; a membrane affixed to the first edge about a perimeter of the frame; and a solidified gel and cell matrix disposed within the bounded area within the frame, wherein the solidified gel and cell matrix substantially fills a volume defined by the bounded area and the height of the frame.

公开(公告)号：US09631173B2

公开(公告)日：2017-04-25

申请号：US14934434

申请日：2015-11-06

Applicant: CPSI Holdings, LLC

Inventor： John G. Baust ,  Joshua T. Smith ,  Kimberly L. Santucci ,  Kristi K. Snyder ,  Anthony T. Robilotto ,  Robert G. Van Buskirk ,  John M. Baust ,  William L. Corwin ,  Jennie F. McKain

IPC: C12N5/00 ,  G01N33/50 ,  C12M3/00 ,  C12M1/12 ,  C12M1/02 ,  C12M1/00 ,  C12N5/071

CPC classification number: C12M21/08 ,  C12M25/06 ,  C12M25/14 ,  C12M27/00 ,  C12M29/00 ,  C12N5/0062 ,  C12N5/0068 ,  C12N5/0697 ,  C12N2537/00 ,  G01N33/5008 ,  G01N33/5017

Abstract: A tissue engineered model (TEM) structure, an apparatus and method for making a TEM structure, and methods of using a TEM structure are disclosed. In an embodiment, the TEM structure includes at least one TEM segment. Each TEM segment includes a frame defining a bounded area, the frame having a height, a first edge, and a second edge opposite the first edge, each of the first edge and the second edge defining a perimeter of the bounded area, and the height defining a distance between the first edge and the second edge; a membrane affixed to the first edge about a perimeter of the frame; and a solidified gel and cell matrix disposed within the bounded area within the frame, wherein the solidified gel and cell matrix substantially fills a volume defined by the bounded area and the height of the frame.

公开(公告)号：US09213025B2

公开(公告)日：2015-12-15

申请号：US14206533

申请日：2014-03-12

Applicant: CPSI HOLDINGS LLC

Inventor： John G. Baust ,  Joshua T. Smith ,  Kimberly L. Santucci ,  Kristi K. Snyder ,  Anthony T. Robilotto ,  Robert G. Van Buskirk ,  John M. Baust ,  William L. Corwin ,  Jennie F. McKain

IPC: C12N5/00 ,  G01N33/50

CPC classification number: C12M21/08 ,  C12M25/06 ,  C12M25/14 ,  C12M27/00 ,  C12M29/00 ,  C12N5/0062 ,  C12N5/0068 ,  C12N5/0697 ,  C12N2537/00 ,  G01N33/5008 ,  G01N33/5017

Abstract: A tissue engineered model (TEM) structure, an apparatus and method for making a TEM structure, and methods of using a TEM structure are disclosed. In an embodiment, the TEM structure includes at least one TEM segment. Each TEM segment includes a frame defining a bounded area, the frame having a height, a first edge, and a second edge opposite the first edge, each of the first edge and the second edge defining a perimeter of the bounded area, and the height defining a distance between the first edge and the second edge; a membrane affixed to the first edge about a perimeter of the frame; and a solidified gel and cell matrix disposed within the bounded area within the frame, wherein the solidified gel and cell matrix substantially fills a volume defined by the bounded area and the height of the frame.

Abstract translation: 公开了一种组织工程模型（TEM）结构，用于制造TEM结构的装置和方法，以及使用TEM结构的方法。 在一个实施例中，TEM结构包括至少一个TEM段。 每个TEM段包括限定有界区域的框架，框架具有高度，第一边缘和与第一边缘相对的第二边缘，第一边缘和第二边缘中的每一个限定有界区域的周边，并且高度 限定所述第一边缘和所述第二边缘之间的距离; 围绕所述框架的周边固定到所述第一边缘的膜; 以及设置在框架内的有界区域内的凝固凝胶和细胞基质，其中凝固的凝胶和细胞基质基本上填充由界限区域和框架的高度限定的体积。