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公开(公告)号：US11155483B1
公开(公告)日：2021-10-26
申请号：US17135984
申请日：2020-12-28
申请人： Nutrition & Health Research Institute, COFCO Corporation , COFCO (jilin) Bio-Chemical Technology CO., Ltd , COFCO BIOTECHNOLOGY CO., LTD. , Tsinghua University
发明人： Yi Tong , Yi Li , Fang Tian , Weiqiang Zhou , Yuanheng Guo , Guoqiang Chen , Dayong Li , Haijun Liu , Kejia Xu , Anni Liu , Bo Chen , Tai An , Xiaoyan Wang , Chao Peng , Xuemei Shen , Jin Tao , Lida Wu , Kai Yang , Qiong Wu
IPC分类号： C02F3/12 , C02F3/34 , C12M1/00 , C12N1/02 , C12N1/06 , C12P7/62 , C12M1/26 , C08G63/90 , B01D37/02 , B01D39/08 , B01D61/00 , C02F1/28 , C02F1/72
摘要： A method for efficiently producing PHA comprising: inoculating PHA fermentation strains into a fermentation medium for fermentation under the condition of being capable of producing PHA through fermentation; subjecting the fermentation broth to a solid-liquid separation to obtain fermentation supernatant and thallus precipitate; breaking the cell walls of the thallus precipitate, and subjecting the wall-broken products to a plate and frame filtration to prepare PHA; pre-coating a filter cloth for the plate and frame filtration with a PHA layer; at least part of the water of the fermentation medium is PHA process wastewater. The method utilizes the PHA process wastewater as at least part of the water of the fermentation medium, and filters and separates the broken thallus with the plate and frame filtration equipment pre-coated with PHA layer to prepare PHA, thereby recycling the high-salt wastewater, reducing costs, and potentially separating PHA on a large scale for industrial production.
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公开(公告)号：US11203663B2
公开(公告)日：2021-12-21
申请号：US17137545
申请日：2020-12-30
申请人： COFCO (jilin) Bio-Chemical Technology CO., Ltd , Nutrition & Health Research Institute, COFCO Corporation , COFCO BIOTECHNOLOGY CO., LTD.
发明人： Yi Tong , Yi Li , Fang Tian , Kejia Xu , Yuanheng Guo , Bo Chen , Tai An , Dayong Li , Haijun Liu , Lida Wu
摘要： Provided a method for separating PHA and PHA prepared therefrom. The method comprises the following steps: subjecting a PHA fermentation broth to solid-liquid separation to obtain a thallus precipitate; breaking cell walls of the thallus precipitate, and subjecting obtained wall-broken products to a plate and frame filtration to obtain PHA; a filter cloth for the plate and frame filtration is pre-coated with a PHA layer. The method adopts a plate and frame separation to replace the traditional centrifugal separation to prepare PHA, and the PHA layer is pre-coated on the filter cloth for the plate and frame filtration, thereby overcome the defects in the prior art such as high cost and operational difficulty caused by adopting multiple centrifugal separations; in addition, the method of the present disclosure also exhibits the advantages of high recovery rate of PHA and high purity of the prepared PHA product.
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公开(公告)号：US20210340316A1
公开(公告)日：2021-11-04
申请号：US17137545
申请日：2020-12-30
申请人： COFCO (jilin) Bio-Chemical Technology CO., Ltd , Nutrition & Health Research Institute, COFCO Corporation , COFCO BIOTECHNOLOGY CO., LTD.
发明人： Yi Tong , Yi Li , Fang Tian , Kejia Xu , Yuanheng Guo , Bo Chen , Tai An , Dayong Li , Haijun Liu , Lida Wu
摘要： Provided a method for separating PHA and PHA prepared therefrom. The method comprises the following steps: subjecting a PHA fermentation broth to solid-liquid separation to obtain a thallus precipitate; breaking cell walls of the thallus precipitate, and subjecting obtained wall-broken products to a plate and frame filtration to obtain PHA; a filter cloth for the plate and frame filtration is pre-coated with a PHA layer. The method adopts a plate and frame separation to replace the traditional centrifugal separation to prepare PHA, and the PHA layer is pre-coated on the filter cloth for the plate and frame filtration, thereby overcome the defects in the prior art such as high cost and operational difficulty caused by adopting multiple centrifugal separations; in addition, the method of the present disclosure also exhibits the advantages of high recovery rate of PHA and high purity of the prepared PHA product.
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