公开(公告)号：US11155483B1

公开(公告)日：2021-10-26

申请号：US17135984

申请日：2020-12-28

申请人： Nutrition & Health Research Institute, COFCO Corporation ,  COFCO (jilin) Bio-Chemical Technology CO., Ltd ,  COFCO BIOTECHNOLOGY CO., LTD. ,  Tsinghua University

发明人： Yi Tong ,  Yi Li ,  Fang Tian ,  Weiqiang Zhou ,  Yuanheng Guo ,  Guoqiang Chen ,  Dayong Li ,  Haijun Liu ,  Kejia Xu ,  Anni Liu ,  Bo Chen ,  Tai An ,  Xiaoyan Wang ,  Chao Peng ,  Xuemei Shen ,  Jin Tao ,  Lida Wu ,  Kai Yang ,  Qiong Wu

IPC分类号： C02F3/12 ,  C02F3/34 ,  C12M1/00 ,  C12N1/02 ,  C12N1/06 ,  C12P7/62 ,  C12M1/26 ,  C08G63/90 ,  B01D37/02 ,  B01D39/08 ,  B01D61/00 ,  C02F1/28 ,  C02F1/72

摘要： A method for efficiently producing PHA comprising: inoculating PHA fermentation strains into a fermentation medium for fermentation under the condition of being capable of producing PHA through fermentation; subjecting the fermentation broth to a solid-liquid separation to obtain fermentation supernatant and thallus precipitate; breaking the cell walls of the thallus precipitate, and subjecting the wall-broken products to a plate and frame filtration to prepare PHA; pre-coating a filter cloth for the plate and frame filtration with a PHA layer; at least part of the water of the fermentation medium is PHA process wastewater. The method utilizes the PHA process wastewater as at least part of the water of the fermentation medium, and filters and separates the broken thallus with the plate and frame filtration equipment pre-coated with PHA layer to prepare PHA, thereby recycling the high-salt wastewater, reducing costs, and potentially separating PHA on a large scale for industrial production.