公开(公告)号：US20240344127A1

公开(公告)日：2024-10-17

申请号：US18632071

申请日：2024-04-10

申请人： CFD Research Corporation

发明人： David Edwin Gaddes, III ,  Kyleigh Raye Jones

IPC分类号： C12Q1/6876 ,  C12Q1/6853

CPC分类号： C12Q1/6876 ,  C12Q1/6853 ,  C12Q2600/16

摘要： A method of performing an asymmetric loop-mediated isothermal amplification (LAMP) assay can include: providing the asymmetrical primer set of one of the embodiments; providing a reagent composition having a polymerase; combining the asymmetrical primer set and reagent composition with a sample having the target nucleic acid to form an amplification mixture; heating the amplification mixture to an amplification temperature within an amplification temperature range; maintaining the amplification temperature to amplify the target nucleic acid having the target sequence; and obtaining an amplified amount of the target nucleic acid having the target sequence. The asymmetrical primer set can include one primer of a primer pair to be at a lower (e.g., 10%) amount of the other primer.