公开(公告)号：US20190010454A1

公开(公告)日：2019-01-10

申请号：US16066389

申请日：2016-07-22

Applicant: CATHOLIC UNIVERSITY INDUSTRY ACADEMIC COOPERATION FOUNDATION

Inventor： So-Hyang CHUNG ,  Hyun Jung LEE ,  Kyung Yul SEO

IPC: C12N5/079 ,  A61L27/36

Abstract: The present invention relates to a method for culturing limbal tissues on an amniotic membrane slide scaffold, thereby enabling the proportion of limbal stem cells in a limbal tissue-derived epithelial cell sheet to be effectively increased, and the same to be cultured. According to the present invention, the proportion of limbal stem cells in in a limbal tissue-derived epithelial cell sheet can be stably and rapidly increased, and thus the success rate can be increased when in limbal tissue-derived epithelial cell sheets are transplanted into a patient with limbal stem cell deficiency.