公开(公告)号：US20180086638A1

公开(公告)日：2018-03-29

申请号：US15725473

申请日：2017-10-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Larry J. Cummings ,  Jie HE

IPC: C01B25/32 ,  C01B25/455 ,  B01D15/20 ,  B01J20/04 ,  B01J20/282 ,  B01J20/34 ,  C07K1/20 ,  C07K1/18 ,  C07K1/22

CPC classification number: C01B25/327 ,  B01D15/203 ,  B01J20/048 ,  B01J20/282 ,  B01J20/3433 ,  B01J20/3475 ,  C01B25/455 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22

Abstract: Methods and compositions are provided for treatment of an apatite-based resin from which retained solutes have been eluted by an elution buffer that contains an alkali metal salt with solutions of calcium ion, phosphate ion, and hydroxide separately from any sample loading and elution buffers. The treatment solutions restore the resin, reversing the deterioration that is caused by the alkali metal salt in the elution buffer.