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公开(公告)号：US20220163549A1
公开(公告)日：2022-05-26
申请号：US17671308
申请日：2022-02-14
Applicant: Beckman Coulter, Inc.
Inventor： Andras Guttman , Clarence Lew , Marton Szigeti , Csaba Varadi
Abstract: The present teachings relate to methods, systems, and kits for the preparation, purification and/or analysis of a glycan or glycoconjugate, and specifically to a magnetic bead based sample preparation protocol. In some aspects, the sample preparation protocol can provide for glycoconjugate capture, glycan release, fluorescent derivatization, and glycan purification for subsequent capillary electrophoresis, liquid chromatography, or other glycoanalytical method using magnetic beads containing negatively charged carboxyl groups extending from the surface of the magnetic beads.
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Patent Agency Ranking
公开(公告)号：US11249098B2
公开(公告)日：2022-02-15
申请号：US16277218
申请日：2019-02-15
Applicant: Beckman Coulter, Inc.
Inventor： Andras Guttman , Clarence Lew , Marton Szigeti , Csaba Varadi
Abstract: The present teachings relate to methods, systems, and kits for the preparation, purification and/or analysis of a glycan or glycoconjugate, and specifically to a magnetic bead based sample preparation protocol. In some aspects, the sample preparation protocol can provide for glycoconjugate capture, glycan release, fluorescent derivatization, and glycan purification for subsequent capillary electrophoresis, liquid chromatography, or other glycoanalytical method using magnetic beads containing negatively charged carboxyl groups extending from the surface of the magnetic beads.
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公开(公告)号：US20170045538A1
公开(公告)日：2017-02-16
申请号：US15307113
申请日：2015-04-27
Applicant: BECKMAN COULTER, INC.
Inventor： Andras Guttman , Clarence Lew , Marton Szigeti , Csaba Varadi
CPC classification number: G01N35/0098 , C08B37/0003 , G01N35/0099 , G01N2035/00356 , G01N2400/12 , G01N2400/38
Abstract: The present teachings relate to methods, systems, and kits for the preparation, purification and/or analysis of a glycan or glycoconjugate, and specifically to a magnetic bead based sample preparation protocol that can enable full automation and reduced sample preparation time relative to current methods of glycoanalysis. In some aspects, the sample preparation protocol can provide for glycoconjugate capture, glycan release, fluorescent derivatization, and glycan purification for subsequent capillary electrophoresis, liquid chromatography, or other glycoanalytical method without requiring time-consuming sample preparation steps such as centrifugation or vacuum-centrifugation.
Abstract translation: 本教导涉及用于制备，纯化和/或分析聚糖或糖缀合物的方法，系统和试剂盒，特别涉及基于磁珠的样品制备方案，其可以实现相对于当前方法的全自动化和减少的样品制备时间 糖分析。 在一些方面，样品制备方案可以提供糖缀合物捕获，聚糖释放，荧光衍生化和聚糖纯化用于随后的毛细管电泳，液相色谱或其它糖分析方法，而不需要耗时的样品制备步骤，例如离心或真空离心 。
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公开(公告)号：US10247745B2
公开(公告)日：2019-04-02
申请号：US15307113
申请日：2015-04-27
Applicant: Beckman Coulter, Inc.
Inventor： Andras Guttman , Clarence Lew , Marton Szigeti , Csaba Varadi
Abstract: The present teachings relate to methods, systems, and kits for the preparation, purification and/or analysis of a glycan or glycoconjugate, and specifically to a magnetic bead based sample preparation protocol that can enable full automation and reduced sample preparation time relative to current methods of glycoanalysis. In some aspects, the sample preparation protocol can provide for glycoconjugate capture, glycan release, fluorescent derivatization, and glycan purification for subsequent capillary electrophoresis, liquid chromatography, or other glycoanalytical method without requiring time-consuming sample preparation steps such as centrifugation or vacuum-centrifugation.
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公开(公告)号：US12210027B2
公开(公告)日：2025-01-28
申请号：US17671308
申请日：2022-02-14
Applicant: Beckman Coulter, Inc.
Inventor： Andras Guttman , Clarence Lew , Marton Szigeti , Csaba Varadi
Abstract: The present teachings relate to methods, systems, and kits for the preparation, purification and/or analysis of a glycan or glycoconjugate, and specifically to a magnetic bead based sample preparation protocol. In some aspects, the sample preparation protocol can provide for glycoconjugate capture, glycan release, fluorescent derivatization, and glycan purification for subsequent capillary electrophoresis, liquid chromatography, or other glycoanalytical method using magnetic beads containing negatively charged carboxyl groups extending from the surface of the magnetic beads.
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公开(公告)号：US20190227086A1
公开(公告)日：2019-07-25
申请号：US16277218
申请日：2019-02-15
Applicant: Beckman Coulter, Inc.
Inventor： Andras Guttman , Clarence Lew , Marton Szigeti , Csaba Varadi
Abstract: The present teachings relate to methods, systems, and kits for the preparation, purification and/or analysis of a glycan or glycoconjugate, and specifically to a magnetic bead based sample preparation protocol that can enable full automation and reduced sample preparation time relative to current methods of glycoanalysis. In some aspects, the sample preparation protocol can provide for glycoconjugate capture, glycan release, fluorescent derivatization, and glycan purification for subsequent capillary electrophoresis, liquid chromatography, or other glycoanalytical method without requiring time-consuming sample preparation steps such as centrifugation or vacuum-centrifugation.
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