公开(公告)号：US10017824B2

公开(公告)日：2018-07-10

申请号：US14908695

申请日：2013-07-30

Applicant: BGI GENOMICS CO., LIMITED

Inventor： Xiaoyu Pan ,  Jing Guo ,  Hui Jiang ,  Fang Chen ,  Shengpei Chen ,  Shida Zhu ,  Fan Fan ,  Jialou Zhu

IPC: C12Q1/6853 ,  C12Q1/6881 ,  C12Q1/6886 ,  C12Q1/6827 ,  C12Q1/6876 ,  C12Q1/6851

CPC classification number: C12Q1/6886 ,  C12Q1/6827 ,  C12Q1/6851 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/154 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2523/125 ,  C12Q2531/113 ,  C12Q2537/143

Abstract: The present invention provides a method for determining the nucleic acid composition in a total nucleic acid mixture comprising a first nucleic acid and a second nucleic acid. The method comprises: 1) treating the total nucleic acid mixture with a bisulfate, to convert the non-methylated cytosine in the total nucleic acid mixture into uracil, and obtain a converted total nucleic acid mixture; 2) subjecting the converted total nucleic acid mixture to multiplexed fluorescent quantitative PCR using a first set of amplification primers and a second set of amplification primers; and 3) based on the ratio R of the methylated amplification product to the non-methylated amplification product of the predetermined nucleic acid fragment, a methylation proportion M1 of the predetermined nucleic acid fragment in the first nucleic acid, and a methylation proportion M2 of the predetermined nucleic acid fragment in the second nucleic acid, determining the nucleic acid composition in the total nucleic acid mixture.