Abstract:
A dispensing apparatus provides a fluid flow path of the recirculating type. A pump provides circulation of fluid within the flow path from a reservoir through a dispenser whose nozzle output may be disposed for dispensing. An actuator is coupled to the dispenser and operates to dispense from the nozzle an accurate measured volume of fluid at times when the pump is inoperative and the fluid within the flow line is otherwise static. Fluid is dispensed upon distortion of the flow line as a result of displacement of surrounding fluid in a closed system.
Abstract:
An automated device for detecting measuring and counting growing microcolonies of bacteria by their light-scattering property. The growing bacteria are contained in glass capillary tubes filled with nutrient agar and are counted by passing the capillary tubes through a narrow beam of light. The tubes are mounted in a holder which is, in turn, detachably secured on a carriage. The carriage is translated in a direction lengthwise of the tubes and then sequentially moved transversely in a step equal to the distance between adjacent tubes. Transverse stepping takes place at the end of the longitudinal travel of each tube. A fixed light source and associated stationary optical means projects a narrow light beam which intersects the tubes as they translate. The presence of growing bacterial microcolonies causes light scattering which is detected in the form of pulses of light by a photomultiplier tube located opposite the path of translation of the tubes relative to the light source. The corresponding signal pulses produced by the photomultiplier tube are counted by an electronic counting circuit which provides a fast count of the particles scanned.