公开(公告)号：US20210115422A1

公开(公告)日：2021-04-22

申请号：US17051937

申请日：2019-04-24

Applicant: BASF SE

Inventor： Asfia Qureshi ,  Adrienne Huston Davenport ,  Xuqiu Tan ,  Oliver Spangenberg ,  Amanda Rae Logue ,  Janina Berndt ,  Tong Li ,  Stefan Seemayer ,  Hans Wolfgang Hoeffken ,  Jingping Zhong

IPC: C12N9/28 ,  A21D8/04

Abstract: Engineered variant polypeptides having amylase enzyme activity, compositions comprising the enzymes, and methods of making and using the enzymes. The genetically engineered amylase enzymes are useful in many different applications such as laundry detergents, dish washing detergents, and cleaning products for homes, industry, vehicle care, baking, animal feed, pulp and paper processing, starch processing, and ethanol production.

公开(公告)号：US11066653B2

公开(公告)日：2021-07-20

申请号：US16631994

申请日：2018-07-11

Applicant: BASF SE

Inventor： Max Fabian Felle ,  Stefan Seemayer

IPC: C12N15/75 ,  C12N9/10

Abstract: The present invention is directed to a producing a DNA methyltransferase in a recombinant host cell, wherein the DNA methyltransferase methylates DNA resulting in a DNA containing 5-methylcytosine within the recognition sequence GCNGC and wherein the DNA methyltransferase comprises less than (35) amino acid residues between amino acid residue (72) and amino acid residue (106) according to the numbering of SEQ ID NO: 33. Furthermore, the present invention is directed to the use of such DNA methyltransferase for the production of bacterial transformants comprising the steps of (a) introducing into a first bacterial host cell a polynucleotide comprising a polynucleotide sequence encoding the DNA methyltransferase to produce a methylated DNA and (b) transferring the methylated DNA from the first bacterial host cell into a second bacterial host cell, wherein the second bacterial host cell comprises a restriction endonuclease able to degrade the DNA but unable to degrade the methylated DNA.

公开(公告)号：US20250034605A1

公开(公告)日：2025-01-30

申请号：US18717729

申请日：2022-12-12

Applicant: BASF SE

Inventor： Gunther Zimmermann ,  Moritz Stefan Pott ,  Michael Breuer ,  Klaus Ditrich ,  Stefan Seemayer

IPC: C12P9/00 ,  A01N57/20 ,  C12N9/80 ,  C12N9/86 ,  C12N9/90 ,  C12P41/00

Abstract: The present invention relates to a method of manufacturing glufosinate, comprising the step of enzymatically cleaving off a carbamoyl moiety of a carbamoyl amino acid compound.

公开(公告)号：US20220259580A1

公开(公告)日：2022-08-18

申请号：US17416480

申请日：2019-12-09

Applicant: BASF SE

Inventor： Kai-Uwe Baldenius ,  Michael Breuer ,  Corinna Ruffer ,  Melanie Weingarten ,  Reinhard Zschoche ,  Stefan Seemayer ,  Bernd Nidetzky ,  Katharina Schmoelzer ,  Michael Puhl

IPC: C12N9/24 ,  C12P19/14 ,  C12P19/18 ,  C12N1/20

Abstract: The present disclosure relates to methods for producing human milk oligosaccharide (HMO) core structures using glycosidases from family GH20 hexosaminidases. In particular, the present disclosure provides methods for producing lacto-N-triose II (LNT II) and/or lacto-N-tetraose (LNT) by reacting glucosamine-oxazoline and/or lacto-N-biose-oxazoline with lactose catalysed by an enzyme of the glycoside hydrolase family 20 (GH20) according to the classification of the Carbohydrate-Active-Enzymes (CAZy) database. Specific optimized enzymes are identified to catalyse the reactions.

公开(公告)号：US12203114B2

公开(公告)日：2025-01-21

申请号：US17286328

申请日：2019-10-09

Applicant: BASF SE

Inventor： Diego Ghislieri ,  Tobias Joachim Zimmermann ,  Stefan Seemayer ,  Michael Breuer ,  Doreen Schachtschabel

IPC: C12N9/78 ,  C12P7/40 ,  C12P7/60

Abstract: Described herein are methods for the production of ammonium acrylate or salts thereof from acrylonitrile using nitrilase as a catalyst.

公开(公告)号：US20230059334A1

公开(公告)日：2023-02-23

申请号：US17785762

申请日：2020-12-17

Applicant: BASF SE

Inventor： Stefan Seemayer ,  Holger Hartmann

IPC: C12Q1/6827 ,  C12N15/10

Abstract: The present invention is concerned with materials and methods for nucleic acid and/or protein analysis, including materials and methods for creating and/or analysing mutant libraries. The invention in particular relates to materials and methods for correlating a property of mutants of a target nucleic acid to the respective sequences of the mutants. The invention is particularly useful for analysing saturation mutagenesis libraries and the analysis of sensitivities to chemical or physical conditions, for example temperature stability and solvent stability, and for the analysis of production properties, for example yield or cellular localisation.

公开(公告)号：US20220017931A1

公开(公告)日：2022-01-20

申请号：US17424824

申请日：2020-01-20

Applicant: BASF SE

Inventor： Diego Ghislieri ,  Christian Willrodt ,  Christopher Koradin ,  Stefan Seemayer ,  Doreen Schachtschabel ,  Kai-Uwe Baldenius ,  Roland Goetz

IPC: C12P13/00 ,  C12N9/78

Abstract: Described herein are methods for the production of 4-cyano benzoic acid or salts thereof from terephthalonitrile using nitrilase as catalyst. Also described herein are compositions including 4-cyano benzoic acid.

公开(公告)号：US20210340518A1

公开(公告)日：2021-11-04

申请号：US17286328

申请日：2019-10-09

Applicant: BASF SE

Inventor： Diego Ghislieri ,  Tobias Joachim Zimmermann ,  Stefan Seemayer ,  Michael Breuer ,  Doreen Schactschabel

IPC: C12N9/78 ,  C12P7/40

Abstract: Described herein are methods for the production of ammonium acrylate or salts thereof from acrylonitrile using nitrilase as a catalyst.

公开(公告)号：US20200165581A1

公开(公告)日：2020-05-28

申请号：US16631994

申请日：2018-07-11

Applicant: BASF SE

Inventor： Max Fabian Felle ,  Stefan Seemayer

IPC: C12N9/10 ,  C12N15/75

Abstract: The present invention is directed to a producing a DNA methyltransferase in a recombinant host cell, wherein the DNA methyltransferase methylates DNA resulting in a DNA containing 5-methylcytosine within the recognition sequence GCNGC and wherein the DNA methyltransferase comprises less than (35) amino acid residues between amino acid residue (72) and amino acid residue (106) according to the numbering of SEQ ID NO: 33. Furthermore, the present invention is directed to the use of such DNA methyltransferase for the production of bacterial transformants comprising the steps of (a) introducing into a first bacterial host cell a polynucleotide comprising a polynucleotide sequence encoding the DNA methyltransferase to produce a methylated DNA and (b) transferring the methylated DNA from the first bacterial host cell into a second bacterial host cell, wherein the second bacterial host cell comprises a restriction endonuclease able to degrade the DNA but unable to degrade the methylated DNA.